Nandakumar M P, Tocaj A, Mattiasson B
Department of Biotechnology, Lund University, Sweden.
Bioseparation. 1999;8(1-5):247-54.
A method for cell disruption in Flow Injection Analysis (FIA) systems has been developed. The principle involves on-line cell disruption by means of immobilised lysozyme followed by an ultrasonic treatment. In order to avoid flow problems in the analytical system, the lysozyme was immobilised to Streamline that was used in an expanded bed in the flow system. Samples of suspensions of Micrococcus lysodeikticus were treated and the success of the treatment was evaluated in terms of released protein and as a decrease in the optical density at 450 nm. The new technology offers a powerful tool in flow injection analyses for quantification of intracellular compounds. The concept of integration, i.e. combining cell disruption with handling of cell debris and assay procedure in one continuous flow process facilitates its use and increases the probability of reaching reproducible and reliable results.
一种用于流动注射分析（FIA）系统的细胞破碎方法已被开发出来。其原理是通过固定化溶菌酶进行在线细胞破碎，随后进行超声处理。为避免分析系统中出现流动问题，将溶菌酶固定在用于流动系统膨胀床的Streamline上。对溶壁微球菌悬浮液样本进行了处理，并根据释放的蛋白质以及450nm处光密度的降低来评估处理的成功与否。这项新技术为流动注射分析中细胞内化合物的定量提供了一个强大的工具。整合的概念，即将细胞破碎与细胞碎片处理及测定程序在一个连续流动过程中相结合，便于其使用，并增加了获得可重复和可靠结果的可能性。
