Bartolome J A, Archbald L F, Morresey P, Hernandez J, Tran T, Kelbert D, Long K, Risco C A, Thatcher W W
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville 32610, USA.
Theriogenology. 2000 Feb;53(3):815-25. doi: 10.1016/S0093-691X(99)00276-9.
The benefit of using timed-insemination in lactating dairy cows for the treatment of ovarian cysts lies in the fact that cows do not have to be detected in estrus for insemination and achieving pregnancy. We compared the effectiveness of synchronization of ovulation with timed-insemination and induction of estrus with insemination at estrus in the treatment of bovine ovarian cysts in lactating dairy cows. After Day 65 post partum, a total of 368 lactating dairy cows was divided into 3 groups. Cows in Group 1 (n = 209, normal, noncystic) were treated with 100 ug, i.m. GnRH on Day 0; 25 mg, i.m. PGF2 alpha on Day 7; and 100 ug, i.m. GnRH on Day 9 and then were time-inseminated 16 h later. Cows in Group 2 (n = 76, abnormal, cystic) were treated with 100 ug, i.m. GnRH on Day 0; 25 mg, i.m. PGF2 alpha on Day 7; and 100 ug, i.m. GnRH on Day 9 and time-inseminated 16 h later. Cows in Group 3 (n = 83, abnormal, cystic) were treated with 100 ug, i.m. GnRH on Day 0; 25 mg, i.m. PGF2 alpha on Day 7; and inseminated at induced estrus within 7 d after treatment with PGF2 alpha. Day 0 was the day of initiation of the study. Conception and pregnancy rates among groups were compared using logistic regression and adjusted for parity, time of year and days in milk. Conception and pregnancy rates of Group 1 cows (31.5%) were not significantly different from those of Group 2 cows (23.6%). However, the pregnancy rate in normal cows (Group 1) was higher (P < 0.01) than in cystic cows (Groups 2 and 3). Cows in Group 3 had a higher conception rate than cows in Group 2 (51.7% > 23.6%; P < 0.01). However, pregnancy rates for cows in Groups 2 (23.6%) and 3 (18%) were not significantly different. The finding indicated that synchronization of ovulation and timed-insemination resulted in pregnancy rates similar to those of synchronization of estrus and insemination at an induced estrus within 7 d for the treatment of ovarian cysts in lactating dairy cows.
在泌乳期奶牛中使用定时输精治疗卵巢囊肿的好处在于，奶牛无需通过发情检测来进行输精和受孕。我们比较了泌乳期奶牛中采用定时输精同步排卵与在发情期输精诱导发情这两种方法治疗牛卵巢囊肿的效果。产后65天后，总共368头泌乳期奶牛被分为3组。第1组（n = 209，正常，非囊肿性）奶牛在第0天肌肉注射100微克促性腺激素释放激素（GnRH）；在第7天肌肉注射25毫克前列腺素F2α（PGF2α）；在第9天肌肉注射100微克GnRH，然后在16小时后进行定时输精。第2组（n = 76，异常，囊肿性）奶牛在第0天肌肉注射100微克GnRH；在第7天肌肉注射25毫克PGF2α；在第9天肌肉注射100微克GnRH，并在16小时后进行定时输精。第3组（n = 83，异常，囊肿性）奶牛在第0天肌肉注射100微克GnRH；在第7天肌肉注射25毫克PGF2α，并在PGF2α治疗后7天内诱导发情时输精。第0天为研究开始日。使用逻辑回归比较各组间的受孕率和妊娠率，并对胎次、年份和产奶天数进行校正。第1组奶牛的受孕率（31.5%）与第2组奶牛的受孕率（23.6%）无显著差异。然而，正常奶牛（第1组）的妊娠率高于囊肿性奶牛（第2组和第3组）（P < 0.01）。第3组奶牛的受孕率高于第2组奶牛（51.7% > 23.6%；P < 0.01）。然而，第2组（23.6%）和第3组（18%）奶牛的妊娠率无显著差异。该研究结果表明，对于泌乳期奶牛卵巢囊肿的治疗，同步排卵和定时输精所获得的妊娠率与同步发情并在诱导发情后7天内输精的妊娠率相似。
