Strauss L, Paranko J, Salmi S, Streng T, Launonen A, Morris N, Lakkakorpi J, Mäkelä S, Santti R
Institute of Biomedicine and Research Laboratory and Department of Biology, University of Turku, Finland.
J Urol. 2000 Apr;163(4):1304-8.
Our previous studies indicate that neonatal estrogenization with diethylstilbestrol (neoDES) of male mice and rats causes partial outlet obstruction. In the present study, type XII and XIV collagens were localized in the bladder to study their role in the development of obstruction.
The bladder sections immunostained with smooth muscle specific a-actin antibody were double labeled either with collagen type XII or type XIV antibodies. The specimens were then analyzed with conventional and confocal fluorescence microscope.
Type XII and XIV collagens were not evenly distributed in the bladder. Further, in neonatally estrogenized rats collagen XIV appeared inside smooth muscle fascicles.
Non-overlapping distributions of collagen XII and XIV suggest their different roles in the urinary bladder. Penetration of collagen XIV inside smooth muscle fascicles may have a role in the development of DES-induced partial outlet obstruction.
我们之前的研究表明，用己烯雌酚对雄性小鼠和大鼠进行新生儿期雌激素化处理（新生期己烯雌酚处理）会导致部分出口梗阻。在本研究中，对膀胱中的 XII 型和 XIV 型胶原蛋白进行定位，以研究它们在梗阻形成过程中的作用。
用平滑肌特异性α-肌动蛋白抗体进行免疫染色的膀胱切片，再用 XII 型或 XIV 型胶原蛋白抗体进行双重标记。然后用传统显微镜和共聚焦荧光显微镜对标本进行分析。
XII 型和 XIV 型胶原蛋白在膀胱中分布不均。此外，在新生期雌激素化的大鼠中，XIV 型胶原蛋白出现在平滑肌束内部。
XII 型和 XIV 型胶原蛋白的非重叠分布表明它们在膀胱中具有不同的作用。XIV 型胶原蛋白渗透到平滑肌束内部可能在己烯雌酚诱导的部分出口梗阻形成过程中发挥作用。
