Maclean J, Campbell S A, Pollock K, Chackrewarthy S, Coggins J R, Lapthorn A J
Department of Chemistry, University of Glasgow, Glasgow G12 8QQ, Scotland.
Acta Crystallogr D Biol Crystallogr. 2000 Apr;56(Pt 4):512-5. doi: 10.1107/s0907444900002377.
Shikimate dehydrogenase from Escherichia coli has been crystallized by the vapour-diffusion method using ammonium sulfate as a precipitant. Mass spectrometry confirmed the purity of the enzyme and dynamic light scattering was used to find the appropriate additives to yield a monodisperse enzyme solution. The crystals are monoclinic, space group C2, with unit-cell parameters a = 110.0, b = 139.8, c = 102.6 A, beta = 122.2 degrees (at 100 K). Native crystals diffract to 2.3 A in-house on a rotating-anode X-ray source. The asymmetric unit is likely to contain four molecules, related by 222 symmetry, corresponding to a packing density of 2.86 A(3) Da(-1).
利用硫酸铵作为沉淀剂，通过气相扩散法使来自大肠杆菌的莽草酸脱氢酶结晶。质谱分析证实了该酶的纯度，并使用动态光散射来寻找合适的添加剂以获得单分散的酶溶液。晶体为单斜晶系，空间群为C2，晶胞参数a = 110.0、b = 139.8、c = 102.6 Å，β = 122.2°（在100 K时）。在旋转阳极X射线源上，天然晶体在内部可衍射至2.3 Å。不对称单元可能包含四个分子，通过222对称相关，对应于2.86 ų Da⁻¹的堆积密度。
