Hagi A, Hayashi H, Kishi K, Wang L, Ebina Y
Division of Molecular Genetics, University of Tokushima, Japan.
J Med Invest. 2000 Feb;47(1-2):19-28.
The chemoattractants, fMLP and PAF, stimulate glucose uptake in phagocytes to obtain an energy source for host defense. Glucose uptake in phagocytes is mainly regulated via glucose transporter type 1 (GLUT1). To examine molecular mechanisms of facilitated glucose uptake in response to fMLP or PAF, we established CHO cells stably expressing fMLP or PAF receptor with c-myc epitope tagged GLUT1 which could immunologically detect GLUT1 on the cell surface. In the CHO cells, both fMLP and PAF directly triggered GLUT1 translocation from the intracellular pool to the cell surface, and stimulated glucose uptake. Therefore, in phagocytes, we propose that fMLP and PAF also trigger GLUT1 translocation to stimulate glucose uptake as an energy source for host defense.
趋化因子N-甲酰甲硫氨酸-亮氨酸-苯丙氨酸（fMLP）和血小板活化因子（PAF）刺激吞噬细胞摄取葡萄糖，以获取宿主防御所需的能量来源。吞噬细胞中的葡萄糖摄取主要通过1型葡萄糖转运蛋白（GLUT1）进行调节。为了研究fMLP或PAF介导的葡萄糖摄取促进作用的分子机制，我们构建了稳定表达fMLP或PAF受体的CHO细胞，其带有c-myc表位标签的GLUT1，可通过免疫方法检测细胞表面的GLUT1。在CHO细胞中，fMLP和PAF均可直接触发GLUT1从细胞内池转运至细胞表面，并刺激葡萄糖摄取。因此，在吞噬细胞中，我们推测fMLP和PAF也会触发GLUT1转运，以刺激葡萄糖摄取，为宿主防御提供能量来源。
