Tong T, Sun H, Liu L
Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing.
Zhonghua Zhong Liu Za Zhi. 1997 Mar;19(2):111-4.
Considerable evidence has showed that apoptosis is involved in both cancer development and inhibition. A new assay (terminal deoxynucleotidyl transferase assay, TdT assay) was recently reported to have advantages in the detection of apoptosis. In this study, this assay was used to investigate antitumor drug-induced apoptosis in human cancer cells.
TdT assay, DNA gel electrophoresis, electron and light microscopy were used to observe apoptosis.
Our results showed that cisplatin-induced apoptosis in both HL-60 and SV40T-transformed human bronchial epithelial cells was detected with a good dose and time response. The occurrence of the apoptosis was preceded by the decrease of bcl-2 mRNA expression. With TdT assay, apoptotic cells were observed in the ovarian tumor of patients treated with carboplatin.
TdT assay may be applicable to moniter apoptosis in human cancers induced by chemotherapy, and to evaluate tumor cell response during treatment.
大量证据表明细胞凋亡与癌症的发生发展及抑制均有关。最近报道了一种新的检测方法（末端脱氧核苷酸转移酶检测法，TdT检测法）在细胞凋亡检测方面具有优势。在本研究中，该检测法被用于研究抗肿瘤药物诱导的人癌细胞凋亡。
采用TdT检测法、DNA凝胶电泳、电子显微镜和光学显微镜观察细胞凋亡。
我们的结果显示，顺铂诱导HL-60细胞和SV40T转化的人支气管上皮细胞凋亡具有良好的剂量和时间反应。凋亡的发生先于bcl-2 mRNA表达的降低。通过TdT检测法，在接受卡铂治疗的患者的卵巢肿瘤中观察到了凋亡细胞。
TdT检测法可能适用于监测化疗诱导的人类癌症中的细胞凋亡，并评估治疗期间肿瘤细胞的反应。
