Ferrol N, Barea J M, Azcón-Aguilar C
Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain.
Curr Genet. 2000 Feb;37(2):112-8. doi: 10.1007/s002940050017.
To identify genes that encode plasma membrane H(+)-ATPases in the arbuscular mycorrhizal fungus Glomus mosseae two sets of degenerate primers matching highly conserved motifs present in all plant and fungal ATPases were designed. Nested PCR-amplification of G. mosseae genomic DNA using the designed degenerate primers was carried out. Sequence analysis of the cloned PCR products identified five different clones (GmHA1, GmHA2, GmHA3, GmHA4 and GmHA5) encoding putative plasma membrane H(+)-ATPases. Comparison of the deduced amino-acid sequences of GmHA1-GmHA5 indicate that GmHA1, GmHA3 and GmHA4 are highly identical, while GmHA2 and GmHA5 are more divergent. The evolutionary and functional significance of the divergence found among the different members of the H(+)-ATPase gene family in G. mosseae is discussed.
为了鉴定编码丛枝菌根真菌摩西球囊霉质膜H(+) -ATP酶的基因，设计了两组与所有植物和真菌ATP酶中高度保守基序匹配的简并引物。使用设计的简并引物对摩西球囊霉基因组DNA进行巢式PCR扩增。对克隆的PCR产物进行序列分析，鉴定出五个不同的克隆（GmHA1、GmHA2、GmHA3、GmHA4和GmHA5），它们编码假定的质膜H(+) -ATP酶。GmHA1 - GmHA5推导氨基酸序列的比较表明，GmHA1、GmHA3和GmHA4高度相同，而GmHA2和GmHA5差异较大。讨论了摩西球囊霉H(+) -ATP酶基因家族不同成员间差异的进化和功能意义。
