Bousso P, Lemaître F, Laouini D, Kanellopoulos J, Kourilsky P
Unité de Biologie Moléculaire du Gène, INSERM U277, Institut Pasteur, 25 rue du Dr Roux, 75015 Paris, France.
Int Immunol. 2000 Apr;12(4):425-30. doi: 10.1093/intimm/12.4.425.
While numerous studies have analyzed the shaping of T cell repertoires by self or foreign peptides, little is known on the influence of commensal self peptides derived from the intestinal flora (IF). Here, we have analyzed naive and immune repertoires in mice devoid of IF [germ-free (GF) mice]. First, by means of an extensive CDR3beta sequencing strategy, we show that the naive peripheral CD8 T cell repertoire does not exhibit a major imprint of IF antigens. Second, using MHC-peptide tetramers, CDR3beta length distribution analyses and TCR sequencing, we show that cytotoxic T lymphocyte (CTL) responses specific for two distinct epitopes are quasi-identical in normal and GF mice. Our findings indicate that, in general, peptides derived from the intestinal microflora have little if any influence on CTL responses in the mouse.
尽管众多研究分析了自身或外来肽对T细胞库的塑造，但对于源自肠道菌群（IF）的共生自身肽的影响却知之甚少。在此，我们分析了无菌（GF）小鼠（即缺乏IF的小鼠）的初始和免疫库。首先，通过广泛的CDR3β测序策略，我们发现初始外周CD8 T细胞库未表现出IF抗原的主要印记。其次，使用MHC-肽四聚体、CDR3β长度分布分析和TCR测序，我们发现正常小鼠和GF小鼠中针对两个不同表位的细胞毒性T淋巴细胞（CTL）反应几乎相同。我们的研究结果表明，一般来说，源自肠道微生物群的肽对小鼠的CTL反应几乎没有影响。
