Kawamoto S, Niwa H, Tashiro F, Sano S, Kondoh G, Takeda J, Tabayashi K, Miyazaki J
Department of Nutrition and Physiological Chemistry, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka, Japan.
FEBS Lett. 2000 Mar 31;470(3):263-8. doi: 10.1016/s0014-5793(00)01338-7.
The success of Cre-mediated conditional gene targeting depends on the specificity of Cre recombinase expression in Cre-transgenic mouse lines. As a tool to evaluate the specificity of Cre expression, we developed a reporter transgenic mouse strain that expresses enhanced green fluorescent protein (EGFP) upon Cre-mediated recombination. We demonstrate that the progeny resulting from a cross between this reporter strain and a transgenic strain expressing Cre in zygotes show ubiquitous EGFP fluorescence. This reporter strain should be useful to monitor the Cre expression directed by various promoters in transgenic mice, including mice in which Cre is expressed transiently during embryogenesis under a developmentally regulated promoter.
Cre介导的条件性基因靶向的成功取决于Cre转基因小鼠品系中Cre重组酶表达的特异性。作为评估Cre表达特异性的工具，我们构建了一种报告基因转基因小鼠品系，该品系在Cre介导的重组后表达增强型绿色荧光蛋白（EGFP）。我们证明，该报告基因品系与在受精卵中表达Cre的转基因品系杂交产生的后代呈现普遍的EGFP荧光。该报告基因品系可用于监测转基因小鼠中各种启动子指导的Cre表达，包括在发育调控启动子控制下在胚胎发育过程中瞬时表达Cre的小鼠。
