在大鼠2-乙酰氨基芴/部分肝切除模型中，肝细胞生长因子基因的体内转移加速了肝卵圆细胞的增殖。
In vivo transfer of hepatocyte growth factor gene accelerates proliferation of hepatic oval cells in a 2-acetylaminofluorene/partial hepatectomy model in rats.
作者信息
Shiota G, Kunisada T, Oyama K, Udagawa A, Nomi T, Tanaka K, Tsutsumi A, Isono M, Nakamura T, Hamada H, Sakatani T, Sell S, Sato K, Ito H, Kawasaki H
机构信息
Second Department of Internal Medicine, Faculty of Medicine, Tottori University, Yonago, Japan.
出版信息
FEBS Lett. 2000 Mar 31;470(3):325-30. doi: 10.1016/s0014-5793(00)01337-5.
To clarify the effect of hepatocyte growth factor (HGF) on proliferation of hepatic oval cells, we transferred HGF gene into liver of the Solt-Farber rat model. Male Fisher 344 rats were infected with a recombinant adenovirus carrying the cDNA for HGF (pAxCAHGF) from tail vein. HGF mRNA showed its peak at 4 days, and diminished thereafter. The total and proliferating cell nuclear antigen-positive hepatic oval cells were significantly elevated in HGF-transferred rats, in which stem cell factor and c-kit mRNA increased at each time point. Our results suggest that in vivo transfer of the HGF gene into liver accelerates proliferation of hepatic oval cells in the Solt-Farber model in rats.
为阐明肝细胞生长因子（HGF）对肝卵圆细胞增殖的影响，我们将HGF基因导入Solt-Farber大鼠模型的肝脏。雄性Fisher 344大鼠经尾静脉感染携带HGF cDNA的重组腺病毒（pAxCAHGF）。HGF mRNA在4天时达到峰值，此后逐渐减少。在转染HGF的大鼠中，总肝卵圆细胞和增殖细胞核抗原阳性的肝卵圆细胞显著增加，其中干细胞因子和c-kit mRNA在每个时间点均增加。我们的结果表明，将HGF基因体内导入大鼠肝脏可加速Solt-Farber模型中肝卵圆细胞的增殖。
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