Johansson M C, Baldetorp B, Oredsson S M
The Jubileum Institute, Department of Oncology, University Hospital, Lund, Sweden.
Anal Cell Pathol. 1999;19(2):91-8. doi: 10.1155/1999/364846.
Energy transfer in flow cytometry can occur when two fluorochromes are bound in close proximity (generally within 100 A) and the emission spectrum of one fluorochrome overlaps significantly with the excitation spectrum of the other. The latter criterium is fulfilled for the fluorochromes fluorescein isothiocyanate and propidium iodide and also the former when they, e.g., are used in bromodeoxyuridine - DNA flow cytometry methods. In the present growth kinetic study using this method, we show that energy transfer does take place between fluorescein isothiocyanate and propidium iodide which results in a detected increase in DNA content with 2-3%. Despite the erroneous increase in the obtained DNA content values, this does not seem to have any influence on the calculation of DNA synthesis time and potential doubling time where the DNA content, based on the relative movement principle of the labelled cells, is used.
当两种荧光染料紧密结合（通常在100埃以内）且一种荧光染料的发射光谱与另一种荧光染料的激发光谱有明显重叠时，流式细胞术中就会发生能量转移。异硫氰酸荧光素和碘化丙啶这两种荧光染料满足后一个条件，并且当它们例如用于溴脱氧尿苷 - DNA流式细胞术方法时也满足前一个条件。在使用该方法的当前生长动力学研究中，我们表明异硫氰酸荧光素和碘化丙啶之间确实发生了能量转移，这导致检测到的DNA含量增加了2 - 3%。尽管获得的DNA含量值出现了错误增加，但这似乎对DNA合成时间和潜在倍增时间的计算没有任何影响，在计算中，基于标记细胞的相对移动原理使用了DNA含量。
