浆液性和黏液性卵巢癌中K-RAS原癌基因的突变及6q27杂合性缺失

Mutation of K-RAS protooncogene and loss of heterozygosity on 6q27 in serous and mucinous ovarian carcinomas.

作者信息

Suzuki M, Saito S, Saga Y, Ohwada M, Sato I

机构信息

Department of Obstetrics and Gynecology, Jichi Medical School, Tochigi, Japan.

出版信息

Cancer Genet Cytogenet. 2000 Apr 15;118(2):132-5. doi: 10.1016/s0165-4608(99)00192-2.

DOI:10.1016/s0165-4608(99)00192-2

PMID:10748293

Abstract

The genetic etiology of serous and mucinous ovarian carcinomas was investigated in 76 affected patients, focusing on the possible loss of heterozygosity (LOH) involving chromosome band 6q27 and K-RAS mutations at codon 12. The incidence of LOH in 6q27 (6q27 LOH) was 41% in 64 informative cases; 53% (20/38) and 23% (6/26) in cases of serous ovarian carcinoma and in those of mucinous ovarian carcinoma, respectively, indicating that the incidence of 6q27 LOH was significantly higher in cases of serous ovarian carcinoma (P < 0.05). The incidence of K-RAS mutations at codon 12 was 23% (15/64); 5% (2/38) and 50% (13/26) in cases of serous ovarian carcinoma and in those of mucinous ovarian carcinoma, respectively, indicating that the incidence of the K-RAS mutations was significantly higher in cases of mucinous ovarian carcinoma (P < 0.0001). Thus, K-RAS mutations at codon 12 and 6q27 LOH were suggested to be involved in the development and/or progression of mucinous ovarian carcinoma and serous ovarian carcinoma, respectively.

摘要

对76例卵巢浆液性癌和黏液性癌患者的遗传病因进行了研究，重点关注6号染色体27区带杂合性缺失（LOH）及密码子12处K-RAS突变的可能性。在64例信息充分的病例中，6q27区带杂合性缺失（6q27 LOH）的发生率为41%；浆液性卵巢癌患者和黏液性卵巢癌患者中的发生率分别为53%（20/38）和23%（6/26），表明浆液性卵巢癌患者中6q27 LOH的发生率显著更高（P<0.05）。密码子12处K-RAS突变的发生率为23%（15/64）；浆液性卵巢癌患者和黏液性卵巢癌患者中的发生率分别为5%（2/38）和50%（13/26），表明黏液性卵巢癌患者中K-RAS突变的发生率显著更高（P<0.0001）。因此，提示密码子12处K-RAS突变和6q27 LOH分别参与了黏液性卵巢癌和浆液性卵巢癌的发生和/或进展。