Haddad G G
Departments of Pediatrics, Section of Respiratory Medicine, and Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
J Appl Physiol (1985). 2000 Apr;88(4):1481-7. doi: 10.1152/jappl.2000.88.4.1481.
Drosophila melanogaster has been used as a genetic model, especially in the past decade, to examine normative biological processes and disease conditions very effectively. These span a wide range of major issues such as aging, cancer, embryogenesis, neural development, apoptosis, and alcohol intoxication. Here, we detail how the Drosophila melanogaster can be used as a genetic model to study the molecular and genetic underpinnings of the response to hypoxia. In our study of the basis of anoxia tolerance, one of the potent approaches that we use is a mutagenesis screen to identify loss-of-function mutants that are anoxia sensitive. The major advantage of this approach is that it is not biased for any particular gene or gene product. Although our screen is in progress, we already have evidence that this approach is useful.
黑腹果蝇一直被用作遗传模型，尤其是在过去十年中，能非常有效地研究正常生物学过程和疾病状况。这些研究涵盖了广泛的重大问题，如衰老、癌症、胚胎发育、神经发育、细胞凋亡和酒精中毒。在这里，我们详细阐述黑腹果蝇如何作为遗传模型来研究对缺氧反应的分子和遗传基础。在我们对缺氧耐受性基础的研究中，我们使用的一种有效方法是诱变筛选，以鉴定对缺氧敏感的功能丧失型突变体。这种方法的主要优点是它对任何特定基因或基因产物都没有偏向性。虽然我们的筛选仍在进行中，但我们已经有证据表明这种方法是有用的。
