Magness R R, Shideman C R, Habermehl D A, Sullivan J A, Bird I M
Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Meriter Hospital/Park 7E, Perinatal Research Laboratories, Madison, WI 53715, USA.
Prostaglandins Other Lipid Mediat. 2000 Mar;60(4-6):103-18. doi: 10.1016/s0090-6980(99)00055-6.
Prostacyclin (PGI(2)) is a potent vasodilator, the level of which is increased during pregnancy, and is the main eicosanoid of which production is elevated in the endothelium and vascular smooth muscle (VSM) of both uterine and omental (systemic) arteries. We tested the hypothesis that during physiologic states that have high uterine blood flow, such as pregnancy and the follicular phase of the ovarian cycle (versus luteal phase and ovariectomized ewes), there is an increased level of prostacyclin synthase (PGIS) expression in ovine uterine and omental artery endothelium and VSM. To investigate this, the cellular localization and PGIS protein expression level in uterine and systemic arteries was examined by immunohistochemistry as well as by Western immunoblot analysis of endothelial-isolated protein and denuded vessels (VSM). Whole uterine, but not omental (systemic), arteries from the pregnant ewes showed an increase (P < 0.001) in PGIS expression. Further localization of PGIS protein by immunohistochemistry and quantification by Western analysis showed PGIS to be somewhat higher in the uterine artery VSM (69 +/- 7%) than endothelium (31 +/- 7%). PGIS protein levels in uterine and omental artery endothelial isolated protein were not altered by ovariectomy or the ovarian cycle, although they were both significantly elevated by pregnancy. Uterine and omental artery VSM PGIS expression levels also were not altered by ovariectomy or the ovarian cycle, whereas PGIS expression, in uterine but not omental artery VSM showed a significant elevation during pregnancy. Thus, the rise in PGI(2) production by uterine arteries observed in ovine pregnancy is paralleled by an elevation in PGIS expression in both endothelium and VSM, whereas those seen in omental arteries is associated with increases in endothelial PGIS.
前列环素（PGI₂）是一种强效血管舒张剂，其水平在孕期升高，并且是子宫动脉和网膜（全身）动脉的内皮及血管平滑肌中生成量增加的主要类花生酸。我们检验了这样一个假设：在子宫血流量高的生理状态下，如妊娠和卵巢周期的卵泡期（相对于黄体期和去卵巢母羊），绵羊子宫和网膜动脉内皮及血管平滑肌中前列环素合酶（PGIS）的表达水平会升高。为了研究这一点，通过免疫组织化学以及对内皮分离蛋白和剥脱血管（血管平滑肌）进行Western免疫印迹分析，检测子宫和全身动脉中PGIS的细胞定位及蛋白表达水平。妊娠母羊的整个子宫动脉而非网膜（全身）动脉显示PGIS表达增加（P < 0.001）。通过免疫组织化学对PGIS蛋白进行进一步定位以及Western分析进行定量显示，PGIS在子宫动脉血管平滑肌中的含量（69 ± 7%）略高于内皮（31 ± 7%）。子宫和网膜动脉内皮分离蛋白中的PGIS蛋白水平不受去卵巢或卵巢周期的影响，尽管二者在妊娠时均显著升高。子宫和网膜动脉血管平滑肌的PGIS表达水平也不受去卵巢或卵巢周期的影响，而子宫动脉血管平滑肌中的PGIS表达在妊娠期间显著升高，网膜动脉则不然。因此，在绵羊妊娠中观察到的子宫动脉PGI₂生成增加与内皮及血管平滑肌中PGIS表达升高相平行，而网膜动脉中PGI₂生成增加则与内皮PGIS增加有关。
