鉴定用连接蛋白43转染的C6胶质瘤细胞中差异表达的基因。

Identification of genes differentially expressed in C6 glioma cells transfected with connexin43.

作者信息

Naus C C, Bond S L, Bechberger J F, Rushlow W

机构信息

Department of Anatomy and Cell Biology, The University of Western Ontario, London, Canada.

出版信息

Brain Res Brain Res Rev. 2000 Apr;32(1):259-66. doi: 10.1016/s0165-0173(99)00087-9.

DOI:10.1016/s0165-0173(99)00087-9

PMID:10751676

Abstract

Astrocytes are characterized by extensive gap junctional intercellular communication (GJIC) mediated primarily by channels composed of connexin43. In contrast, C6 glioma cells are deficient in connexin expression and gap junctional communication. Transfection of these glioma cells with connexin cDNAs results in changes in cellular phenotype following increased GJIC. Specifically, connexin expression correlates with reduced cellular proliferation and tumorigenicity. To characterize the role of gap junctions in this growth control, we have screened for changes in gene expression by differential display. We have observed that these changes in GJIC are associated with changes in expression of several genes, including those coding for a number of secreted factors which may play a role in modulating the tumor phenotype of these cells. These include the immediate early gene cyr61, ostoepontin and the KC gene (murine homologue of the human gro gene).

摘要

星形胶质细胞的特征是广泛的间隙连接细胞间通讯（GJIC），主要由连接蛋白43组成的通道介导。相比之下，C6胶质瘤细胞缺乏连接蛋白表达和间隙连接通讯。用连接蛋白cDNA转染这些胶质瘤细胞会导致GJIC增加后细胞表型发生变化。具体而言，连接蛋白表达与细胞增殖减少和致瘤性降低相关。为了表征间隙连接在这种生长控制中的作用，我们通过差异显示筛选了基因表达的变化。我们观察到，这些GJIC的变化与几个基因的表达变化相关，包括那些编码一些分泌因子的基因，这些因子可能在调节这些细胞的肿瘤表型中发挥作用。这些因子包括即刻早期基因cyr61、骨桥蛋白和KC基因（人类gro基因的小鼠同源物）。