Anakina R P, Drozdov A L
Zoological Institute, Russian Academy of Sciences, St. Petersburg.
Tsitologiia. 2000;42(2):128-35.
Ovogenesis of the Barents sea sponge Leucosolenia complicata Mont. having asconoid canal system was studied at light (using histochemical methods) and ultrastructural levels. It has been established that the reproduction period "arranges" for autumn-winter season. The ovogenesis lasts for approximately two months. Grand accroissement is the longest stage of ovogenesis, lastiing in L. complicata from the end of Sptember to the middle of November. At this stage ovocytes lose their amebic movement, which coincides with the beginning of nurse cell formation. The nurse cells are derived from choanocytes. The choanocytes contacting with the ovocyte surface lose the collar and flagellum, grow in size and transform into nurse cells. Any cell of nurse-cell complex is capable of seizing a spermium and being transformed in situ into a carrier-cell. The specific role of nurse-cell complex is to synthesize sudanophilic (lipid) granules for the carrier cell and ovocyte. In the whole, a weak phagocytic activity of ovocytes, is characteristic of the ovogenesis of L. complicata. During vitellogenesis the ovocyte synthesises endogenic mucopolysaccharide granules from oligosaccharides originated from the mesohyl. Lipid granules pass to the ovocyte from nurse cells. Ribosomal RNAs are accumulated as a result of the own activity of nucleolus-nucleus apparatus of the ovocyte.
对具有单沟型管道系统的巴伦支海海绵复杂白枝海绵（Leucosolenia complicata Mont.）的卵子发生过程进行了光镜（使用组织化学方法）和超微结构水平的研究。已确定繁殖期为秋冬季节。卵子发生持续约两个月。大生长期是卵子发生最长的阶段，在复杂白枝海绵中从9月底持续到11月中旬。在此阶段，卵母细胞失去其阿米巴运动，这与营养细胞形成的开始相吻合。营养细胞来源于领细胞。与卵母细胞表面接触的领细胞失去领和鞭毛，体积增大并转化为营养细胞。营养细胞复合体的任何细胞都能够捕获精子并就地转化为携带细胞。营养细胞复合体的特定作用是为携带细胞和卵母细胞合成嗜苏丹性（脂质）颗粒。总体而言，卵母细胞的吞噬活性较弱是复杂白枝海绵卵子发生的特征。在卵黄发生过程中，卵母细胞从源自中胶层的寡糖合成内源性粘多糖颗粒。脂质颗粒从营养细胞传递到卵母细胞。核糖体RNA是卵母细胞的核仁-细胞核装置自身活动积累的结果。
