Kanda I, Akazome Y, Ogasawara O, Mori T
Department of Biological Sciences, Graduate School of Science, University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan.
Gen Comp Endocrinol. 2000 Apr;118(1):96-104. doi: 10.1006/gcen.1999.7448.
Expression of cytochrome P450 cholesterol side chain cleavage (P450scc) and 3beta-hydroxysteroid dehydrogenase (3beta-HSD) mRNAs was examined in chicken embryonic adrenal glands and gonads between days 4 and 12 of incubation. In situ hybridization analysis showed that 3beta-HSD mRNA appeared on day 5 of incubation in the adrenal glands and on day 6 in the gonads, while P450scc mRNA was expressed on day 7 in both the adrenal glands and the gonads. Cells expressing both enzyme mRNAs were distributed in the steroidogenic tissues of the adrenal glands and in the medullary cords of the gonads. From days 9 to 11 of incubation, P450scc mRNA expression was not found in the majority of both the adrenal glands and the gonads, but was detected again in both on day 12, although 3beta-HSD mRNA was constitutively expressed during this period. Changes in the expression pattern of P450scc mRNA are paralleled by changes in the plasma corticosterone level reported previously. Therefore, it is suggested that P450scc is essential to embryogenesis.
在孵化第4天至12天期间，检测了鸡胚肾上腺和性腺中细胞色素P450胆固醇侧链裂解酶（P450scc）和3β-羟基类固醇脱氢酶（3β-HSD）mRNA的表达。原位杂交分析表明，3β-HSD mRNA在孵化第5天出现在肾上腺中，在第6天出现在性腺中，而P450scc mRNA在第7天在肾上腺和性腺中均有表达。表达这两种酶mRNA的细胞分布在肾上腺的类固醇生成组织和性腺的髓索中。在孵化第9天至11天期间，大多数肾上腺和性腺中均未发现P450scc mRNA表达，但在第12天在两者中再次检测到，尽管在此期间3β-HSD mRNA持续表达。P450scc mRNA表达模式的变化与先前报道的血浆皮质酮水平的变化平行。因此，提示P450scc对胚胎发育至关重要。
