Fan L, Reilly C R, Luo Y, Dorf M E, Lo D
Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA.
J Immunol. 2000 Apr 15;164(8):3955-9. doi: 10.4049/jimmunol.164.8.3955.
To test whether accumulation of naive lymphocytes is sufficient to trigger lymphoid development, we generated mice with islet expression of the chemokine TCA4/SLC. This chemokine is specific for naive lymphocytes and mature dendritic cells (DC) which express the CCR7 receptor. Islets initially developed accumulations of T cells with DC, with scattered B cells at the perimeter. These infiltrates consolidated into organized lymphoid tissue, with high endothelial venules and stromal reticulum. Infiltrate lymphocytes showed a naive CD44low CD25- CD69- phenotype, though half were CD62L negative. When backcrossed to RAG-1 knockout, DC were not recruited. Interestingly, islet lymphoid tissue developed in backcrosses to Ikaros knockout mice despite the absence of normal peripheral nodes. Our results indicate that TCA4/SLC can induce the development and organization of lymphoid tissue through diffential recruitment of T and B lymphocytes and secondary effects on stromal cell development.
为了测试幼稚淋巴细胞的积累是否足以触发淋巴组织发育，我们构建了在胰岛中表达趋化因子TCA4/SLC的小鼠。这种趋化因子对表达CCR7受体的幼稚淋巴细胞和成熟树突状细胞（DC）具有特异性。胰岛最初出现了T细胞与DC的聚集，周边有散在的B细胞。这些浸润细胞整合形成有组织的淋巴组织，伴有高内皮微静脉和基质网状结构。浸润的淋巴细胞表现出幼稚的CD44low CD25- CD69-表型，尽管其中一半为CD62L阴性。当回交到RAG-1基因敲除小鼠时，DC未被招募。有趣的是，尽管没有正常的外周淋巴结，但在回交到Ikaros基因敲除小鼠时胰岛淋巴组织仍发育形成。我们的结果表明，TCA4/SLC可通过差异性招募T和B淋巴细胞以及对基质细胞发育的继发性影响来诱导淋巴组织的发育和组织化。
