Kawanami O, Jin E, Ghazizadeh M, Fujiwara M, Jiang L, Nagashima M, Shimizu H, Takemura T, Ohaki Y, Arai S, Gomibuchi M, Takeda K, Yu Z X, Ferrans V J
Department of Molecular Pathology, Institute of Gerontology, Nippon Medical School, Japan. kawanami/
J Nippon Med Sch. 2000 Apr;67(2):118-25. doi: 10.1272/jnms.67.118.
Laser scanning confocal fluorescence microscopy techniques were used to study the localization of von Willebrand factor (vWf; Factor VIII-related antigen) and thrombomodulin (transmembrane receptor for thrombin) in the microvascular endothelial cells in the normal human lung. Tissues were obtained from lobectomy specimens resected for solitary nodules (7 adenocarcinomas and 4 hamartomas) from 11 patients. The plasma membranes of the capillary endothelial cells in the alveolar zones (A-zones) showed red linear fluorescence for thrombomodulin. However, their cytoplasm was mostly unreactive for vWf. The microvessels which were located in the connective tissue (C-zones), including peribronchial, and subpleural areas and large vascular walls, consistently demonstrated band-like green fluorescence for vWf in their cytoplasm, and their plasma membranes usually lacked reactivity for thrombomodulin. Only a limited number of peribronchial capillaries measuring <10 microm in diameter showed a mosaic-like appearance, in which red fluorescence along the plasma membranes was found together with green fluorescence in the subjacent cytoplasm. In the juxtaalveolar (J-zones) microvessels located along the borders between A- and C-zones, and measuring up to 40 microm in diameter, the endothelial cells showed a mosaic-like pattern of distribution of the two antigens. However, the localization of thrombomodulin in the J-zone microvessels was separate and independent from that of vWf. The thrombomodulin-reactive cells were directly connected to the alveolar capillary endothelial cells. Heterogeneous patterns of distribution of thrombomodulin and vWf suggest that topographic differences of endothelial function occur to maintain a balance of coagulation and anticoagulation in the normal human lung.
采用激光扫描共聚焦荧光显微镜技术研究血管性血友病因子（vWf；VIII因子相关抗原）和血栓调节蛋白（凝血酶的跨膜受体）在正常人类肺组织微血管内皮细胞中的定位。组织取自11例因孤立性结节（7例腺癌和4例错构瘤）行肺叶切除术的标本。肺泡区（A区）毛细血管内皮细胞的质膜显示血栓调节蛋白呈红色线性荧光。然而，它们的细胞质对vWf大多无反应。位于结缔组织（C区）的微血管，包括支气管周围、胸膜下区域和大血管壁，其细胞质中始终显示vWf呈带状绿色荧光，其质膜通常对血栓调节蛋白无反应。只有少数直径<10微米的支气管周围毛细血管呈现马赛克样外观，其中质膜上的红色荧光与相邻细胞质中的绿色荧光同时出现。在沿A区和C区边界分布、直径达40微米的近肺泡（J区）微血管中，内皮细胞显示出两种抗原的马赛克样分布模式。然而，J区微血管中血栓调节蛋白的定位与vWf的定位是分开且独立的。血栓调节蛋白反应性细胞直接与肺泡毛细血管内皮细胞相连。血栓调节蛋白和vWf的异质性分布模式表明，内皮功能的地形差异在正常人类肺组织中维持凝血和抗凝平衡。
