Thomas C M, Tang S, Hammond-Kosack K, Jones J D
Sainsbury Laboratory, John Innes Centre, Norwich Research Park, UK.
Mol Plant Microbe Interact. 2000 Apr;13(4):465-9. doi: 10.1094/MPMI.2000.13.4.465.
We have previously shown that tomato Cf-9 induces an Avr9-dependent hypersensitive response (HR) in Nicotiana tabacum and potato. We show here that Cf-4 also induces an Avr4-dependent HR in two tobacco species (N. tabacum and N. benthamiana). The HR induced by Cf-4 and Cf-9 was compared in stable tobacco transgenics by a seedling lethal assay and resistance to recombinant Potato virus X expressing Avr4 or Avr9. We also compared HR induction with Agrobacterium-mediated transient expression. The Cf-4/Avr4 combination induced a more rapid HR than Cf-9/Avr9. Sensitive assays for Cf-9 and Cf-4 function should prove useful for structure/function analyses of these resistance proteins in tobacco.
我们之前已经表明，番茄Cf-9在烟草和马铃薯中诱导了依赖Avr9的过敏反应（HR）。我们在此表明，Cf-4在两种烟草品种（烟草和本氏烟草）中也诱导了依赖Avr4的HR。通过幼苗致死试验以及对表达Avr4或Avr9的重组马铃薯X病毒的抗性，在稳定的烟草转基因植株中比较了Cf-4和Cf-9诱导的HR。我们还通过农杆菌介导的瞬时表达比较了HR诱导情况。Cf-4/Avr4组合诱导的HR比Cf-9/Avr9更快。对Cf-9和Cf-4功能的灵敏检测对于烟草中这些抗性蛋白的结构/功能分析应是有用的。
