Magata Y, Kitano H, Shiozaki T, Iida Y, Nishizawa S, Saji H, Konishi J
Department of Nuclear Medicine and Diagnostic Imaging, Graduate School of Medicine, Kyoto University, Japan.
Nucl Med Biol. 2000 Jan;27(1):57-60. doi: 10.1016/s0969-8051(99)00078-5.
The purpose of this study was to clarify the effect of (-)-nicotine on cerebral benzodiazepine receptors (BzR) with radiotracer methods. The effect of (-)-nicotine on BzR was examined in in vitro studies using chronic (-)-nicotine-treated rats using 3H-diazepam. The in vitro radioreceptor assay showed a 14% increase in the maximum number of binding sites of BzR in chronic (-)-nicotine-treated rats in comparison with the control rats. Moreover, a convenient in vivo uptake index of 125I-iomazenil was calculated and a higher uptake of the radioactivity was observed in the chronic (-)-nicotine-treated group than in the control group. Although further studies of the mechanism of (-)-nicotine on such BzR changes are required, an increase in the amount of BzR in the cerebral cortex was found in rats that underwent chronic (-)-nicotine treatment, and this result contributed to the understanding of the effects of (-)-nicotine and smoking on neural functions.
本研究的目的是用放射性示踪剂方法阐明（-）-尼古丁对脑苯二氮䓬受体（BzR）的影响。在体外研究中，使用3H-地西泮对慢性（-）-尼古丁处理的大鼠进行研究，以检测（-）-尼古丁对BzR的影响。体外放射受体分析显示，与对照大鼠相比，慢性（-）-尼古丁处理的大鼠中BzR结合位点的最大数量增加了14%。此外，计算了125I-异喹胍方便的体内摄取指数，观察到慢性（-）-尼古丁处理组的放射性摄取高于对照组。虽然需要进一步研究（-）-尼古丁对这种BzR变化的作用机制，但在接受慢性（-）-尼古丁处理的大鼠中发现大脑皮质中BzR的数量增加，这一结果有助于理解（-）-尼古丁和吸烟对神经功能的影响。
