Choi J, Appella E, Donehower L A
Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030, USA.
Genomics. 2000 Mar 15;64(3):298-306. doi: 10.1006/geno.2000.6134.
The human wildtype p53-induced phosphatase 1 (Wip1; GenBank symbol Ppm1d) gene encodes a type 2C protein phosphatase (PP2C) that is induced by ionizing radiation in a p53-dependent manner. We have cloned and sequenced the mouse Wip1 gene and its encoded mRNA. The mouse Wip1 gene is composed of six exons and spans over 36 kb of DNA. The mouse cDNA sequence predicts a 598-amino-acid protein with a molecular mass of roughly 66 kDa. Comparison of human and mouse Wip1 sequences revealed 83% overall identity at the amino acid level. The 5'-flanking region of exon 1 had promoter elements characteristic of a housekeeping gene. The Wip1 coding sequences share conserved functional regions with other PP2Cs from a diverse array of species. Expression of Wip1 mRNA was detected ubiquitously in adult and embryonic tissues, though expression in the testis was much higher than in other tissues. Wip1 has been mapped near the p53 gene on mouse chromosome 11.
人类野生型p53诱导的磷酸酶1（Wip1；基因库符号Ppm1d）基因编码一种2C型蛋白磷酸酶（PP2C），该酶以p53依赖的方式由电离辐射诱导产生。我们已克隆并测序了小鼠Wip1基因及其编码的mRNA。小鼠Wip1基因由六个外显子组成，跨越超过36 kb的DNA。小鼠cDNA序列预测出一个由598个氨基酸组成的蛋白质，分子量约为66 kDa。人和小鼠Wip1序列的比较显示，氨基酸水平上总体一致性为83%。外显子1的5'侧翼区域具有管家基因特有的启动子元件。Wip1编码序列与来自多种物种的其他PP2C共享保守的功能区域。在成年和胚胎组织中均普遍检测到Wip1 mRNA的表达，不过在睾丸中的表达远高于其他组织。Wip1已被定位在小鼠11号染色体上靠近p53基因的位置。
