Ueno T, Ohtawa K, Kimoto Y, Sakurai K, Kodera Y, Hiroto M, Matsushima A, Nishimura H, Inada Y
Toin Human Science and Technology Center, Department of Biomedical Engineering, Toin University of Yokohama, Japan.
Cancer Detect Prev. 2000;24(1):100-6.
The jack bean lectin, concanavalin A (Con A), was modified with 2,4-bis[O-methoxypoly(ethylene glycol)]-6-chloro-s-triazine, activated PEG2, to form PEG-Con A. The immunoreactivity of PEG-Con A towards anti-Con A antibodies was reduced by increasing the degree of modification of amino groups in the Con A molecule. PEG-Con A had a complete reduction of the immunogenicity in mice and prolonged the clearance-time in blood. Although the mitogenic activity of Con A towards murine spleen cells was reduced by the conjugation with activated PEG2, the administration of PEG-Con A to mice enhanced the anti-tumor cytotoxicity of peripheral lymphocytes against melanoma B16 cells.
刀豆球蛋白A（Con A），即杰克豆凝集素，用2,4-双[O-甲氧基聚（乙二醇）]-6-氯-s-三嗪（活性聚乙二醇2，activated PEG2）进行修饰，形成聚乙二醇化刀豆球蛋白A（PEG-Con A）。通过提高Con A分子中氨基的修饰程度，PEG-Con A对抗Con A抗体的免疫反应性降低。PEG-Con A在小鼠体内的免疫原性完全降低，且延长了血液中的清除时间。尽管Con A与活性PEG2偶联后对小鼠脾细胞的促有丝分裂活性降低，但给小鼠注射PEG-Con A可增强外周淋巴细胞对黑色素瘤B16细胞的抗肿瘤细胞毒性。
