Weiner M W
Science. 1979 Apr 13;204(4389):187-8. doi: 10.1126/science.107585.
In studies with toad bladders, the uncoupling agent 2,4-dinitrophenol (DNP) reversed the inhibition of CO2 production produced by direct inhibition of transport. In contrast, DNP did not reverse the inhibition of CO2 production brought about by metabolic inhibitors. Therefore, the response to DNP distinguished between inhibition of transport and metabolism; this approach may be useful for the investigation of factors that regulate active transport.
在对蟾蜍膀胱的研究中，解偶联剂2,4-二硝基苯酚（DNP）逆转了因直接抑制转运而产生的二氧化碳产生抑制作用。相比之下，DNP并没有逆转代谢抑制剂所导致的二氧化碳产生抑制作用。因此，对DNP的反应区分了转运抑制和代谢抑制；这种方法可能有助于研究调节主动转运的因素。
