Baudena M A, Chapman M R, Larsen M, Klei T R
Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Vet Parasitol. 2000 Apr 28;89(3):219-30. doi: 10.1016/s0304-4017(00)00201-6.
The effectiveness of Duddingtonia flagrans in reducing the free living third stage larvae (L(3)) of equine cyathostomes on pasture when fed to horses has been demonstrated in cold temperate climates. The objective of this experiment was to assess the efficacy of D. flagrans against equine cyathostomes in the subtropical environment of southern Louisiana. Fecal pats were prepared by mixing feces obtained from a parasite-free horse fed D. flagrans at a dose of approximately 2 x 10(6) spores kg(-1), with feces containing cyathostome eggs from a parasitized horse. Control pats contained feces from a parasite-free horse mixed with feces containing cyathostome eggs. The fecal pats were placed on pasture in six replicates at 4-week intervals from March 1997 until January 1998. Comparison of recoveries of L(3) from non-treated control pats in the field with non-treated coprocultures maintained in the laboratory indicated that L(3) survival on pasture was reduced during the months of May, June, July, August and September. The efficacy of the fungus was determined by L(3) recovery from grass surrounding the fecal pats of treated and control groups. D. flagrans significantly reduced L(3) during the months of April, May, and October 1997 to January 1998 (range 66-99% reduction, p=0.0001), and for the year as a whole (p=0.0001).
在寒冷温带气候条件下，已证实将弗氏网尾线虫投喂给马时，其在降低牧场上马圆线虫自由生活的第三期幼虫（L(3)）数量方面具有有效性。本实验的目的是评估弗氏网尾线虫在路易斯安那州南部亚热带环境中对马圆线虫的防治效果。通过将从以约2×10(6)个孢子/千克剂量投喂弗氏网尾线虫的无寄生虫马获取的粪便，与来自感染马的含有马圆线虫卵的粪便混合，制备粪便堆。对照粪便堆包含来自无寄生虫马的粪便与含有马圆线虫卵的粪便的混合物。从1997年3月至1998年1月，每隔4周将粪便堆放置在牧场上，共设六个重复。将田间未处理的对照粪便堆中的L(3)回收率与实验室中保存的未处理粪便培养物进行比较，结果表明，在5月、6月、7月、8月和9月期间，牧场上L(3)的存活率有所降低。通过从处理组和对照组粪便堆周围的草中回收L(3)来确定该真菌的防治效果。在1997年4月、5月以及1997年10月至1998年1月期间，弗氏网尾线虫显著降低了L(3)的数量（降低幅度为66% - 99%，p = 0.0001），并且在全年整体上也有显著效果（p = 0.0001）。
