Larsen M, Nansen P, Grøndahl C, Thamsborg S M, Grønvold J, Wolstrup J, Henriksen S A, Monrad J
Danish Centre for Experimental Parasitology, Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.
Parasitology. 1996 Jul;113 ( Pt 1):1-6. doi: 10.1017/s003118200006621x.
A field trial was conducted to evaluate the potential of the nematode-destroying fungus Duddingtonia flagrans to control free-living stages of horse strongyles. In late Spring 2 groups of horses (yearlings) with mixed infections of strongyles were allowed to contaminate 2 equal-sized pastures. One of the groups (F) received a daily dose of D. flagrans mixed in a feed supplement, while the other (C) received a similar amount of supplement without fungus. During a 3-month contamination period strongyle egg counts in faeces and number of infective strongyle larvae harvested from faecal cultures were determined. Grass samples were collected fortnightly. After the contamination period the yearlings were removed and 2 groups of young tracer foals (TF and TC) grazed the fungus and control pastures respectively for 4 weeks, housed for another 15 weeks and then killed to determine their worm burdens. The number of larvae in cultures from group TF was significantly lower than that in TC and herbage infectivity was reduced to a very low level on the pasture grazed by horses fed fungi. The number of Strongylus vulgaris and Strongylus edentatus larvae was also significantly lowered in group TF. Cyathostome larvae recovered from the mucosa of the ventral and dorsal colon and from the caecum were significantly lowered in group TF foals. Also, the number of strongyles found in the gut contents of group TF foals were significantly reduced in the dorsal colon, but numbers of worms in the ventral colon and in the caecum were similar to those of the controls.
进行了一项田间试验，以评估线虫寄生真菌弗氏新拟青霉（Duddingtonia flagrans）控制马圆线虫自由生活阶段的潜力。在晚春时节，将两组感染混合圆线虫的一岁马放入两个面积相等的牧场中，使其污染牧场。其中一组（F组）每日在饲料补充剂中混入一定剂量的弗氏新拟青霉，另一组（C组）则给予等量不含真菌的补充剂。在为期3个月的污染期内，测定粪便中的圆线虫卵计数以及从粪便培养物中收获的感染性圆线虫幼虫数量。每两周采集一次草样。污染期结束后，将一岁马移走，两组幼龄示踪马驹（TF组和TC组）分别在接种真菌的牧场和对照牧场放牧4周，再圈养15周，然后宰杀以确定它们的蠕虫负荷。TF组培养物中的幼虫数量显著低于TC组，并且在喂食真菌的马所放牧的牧场上，牧草的感染性降至非常低的水平。TF组普通圆线虫（Strongylus vulgaris）和无齿圆线虫（Strongylus edentatus）幼虫的数量也显著降低。从TF组马驹的腹侧结肠和背侧结肠黏膜以及盲肠中回收的杯状圆线虫幼虫数量显著降低。此外，在TF组马驹的肠道内容物中，背侧结肠中发现的圆线虫数量显著减少，但腹侧结肠和盲肠中的蠕虫数量与对照组相似。
