Smania A M, Saleh M C, Argaraña C E
Centro de Investigaciones en Química Biológica de Córdoba-CONICET, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina.
Genetica. 1999;105(3):233-8. doi: 10.1023/a:1003817311179.
A sequence similar to prokaryotic transposable elements was identified in the long 5' untranslated region (5'UTR) of the butanediol dehydrogenase cDNA isolated from a bovine brain lambda gt11 library. Several observations suggested that this sequence could be related to bacterial IS elements: (a) 58% nucleotide sequence identity, (b) 56% amino acid sequence identity, and (c) the presence of inverted terminal repeats. However, nucleotide sequence analyses of the 5'UTR bovine cDNA showed the presence of chain-terminating nucleotide substitutions that would render it incapable of encoding a functional transposase. Finally, it was observed that different vertebrate genomes have sequences related to this putative transposable element.
在从牛脑λgt11文库中分离出的丁二醇脱氢酶cDNA的长5'非翻译区（5'UTR）中，鉴定出了一段与原核转座元件相似的序列。多项观察结果表明，该序列可能与细菌插入序列（IS元件）相关：（a）核苷酸序列同一性为58%，（b）氨基酸序列同一性为56%，以及（c）存在反向末端重复序列。然而，对5'UTR牛cDNA的核苷酸序列分析表明，存在链终止核苷酸取代，这将使其无法编码功能性转座酶。最后，观察到不同的脊椎动物基因组具有与这种假定的转座元件相关的序列。
