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简短报告:人类可能感染微小隐孢子虫

Short report: possible Cryptosporidium muris infection in humans.

作者信息

Katsumata T, Hosea D, Ranuh I G, Uga S, Yanagi T, Kohno S

机构信息

Department of Parasitology, Institute of Tropical Medicine, Nagasaki University, Japan.

出版信息

Am J Trop Med Hyg. 2000 Jan;62(1):70-2. doi: 10.4269/ajtmh.2000.62.70.

Abstract

Oocysts of cryptosporidia whose morphology resembled that of Cryptosporidium muris were found in the stool of 2 healthy girls in Surabaya, Indonesia. The oocysts were predominantly oval and measured 7.75+/-0.17 x 5.55+/-0.13 microm (mean+/-SD). The number of oocysts excreted were more than 10(5) per gram of stool. The oocysts were well stained with fluorescein-conjugated monoclonal antibody to Cryptosporidium. The specimens from both girls containing the oocysts showed a positive result by the polymerase chain reaction (PCR) using primers specific for the genus Cryptosporidium, but a negative result by the PCR using primers specific for C. parvum. The 2 girls passed oocysts for 5 and 6 days, respectively. They did not complain of any symptoms during the passage of oocysts.

摘要

在印度尼西亚泗水的2名健康女孩的粪便中发现了隐孢子虫的卵囊,其形态与鼠隐孢子虫相似。卵囊主要呈椭圆形,大小为7.75±0.17×5.55±0.13微米(平均值±标准差)。每克粪便中排出的卵囊数量超过10⁵个。这些卵囊用针对隐孢子虫的荧光素偶联单克隆抗体染色效果良好。两名女孩含有卵囊的标本,使用针对隐孢子虫属的引物进行聚合酶链反应(PCR)时呈阳性结果,但使用针对微小隐孢子虫的引物进行PCR时呈阴性结果。这两名女孩分别排出卵囊5天和6天。在排出卵囊期间,她们没有诉说任何症状。

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