Kodádková A, Kvác M, Ditrich O, Sak B, Xiao L
Faculty of Science, University of South Bohemia, Branisovská 31, 370 05 Ceské Budejovice, Czech Republic.
J Parasitol. 2010 Feb;96(1):211-2. doi: 10.1645/GE-2212.1.
Cryptosporidium spp. infection in captive exotic mammals was investigated using staining and molecular biological methods. A total of 323 fecal samples from 100 mammalian species (62 Artiodactyla, 33 Rodentia, 3 Perissodactyla, and 2 Paenungultata) in 4 zoological gardens in the Czech Republic was examined. Only in a reticulated giraffe (Giraffa camelopardalis reticulata) sample was Cryptosporidium sp. infection detected. The partial small subunit rRNA sequence obtained from the isolate was identical to sequences of Cryptosporidium muris in rock hyrax (Procavia capensis) and Bactrian camel (Camelus bactrianus). Neonatal BALB/c mice inoculated with 1 x 10(3) fresh oocysts of the C. muris giraffe isolate did not produce a detectable infection.
采用染色和分子生物学方法对圈养外来哺乳动物中的隐孢子虫属感染情况进行了调查。对捷克共和国4个动物园中100种哺乳动物（62种偶蹄目、33种啮齿目、3种奇蹄目和2种蹄兔目）的323份粪便样本进行了检测。仅在一只网纹长颈鹿（Giraffa camelopardalis reticulata）的样本中检测到隐孢子虫属感染。从该分离株获得的部分小亚基rRNA序列与岩蹄兔（Procavia capensis）和双峰驼（Camelus bactrianus）中的鼠隐孢子虫（Cryptosporidium muris）序列相同。用1×10(3)个长颈鹿鼠隐孢子虫分离株的新鲜卵囊接种新生BALB/c小鼠，未产生可检测到的感染。
