德国小蠊核糖体DNA簇的种内多态性分析。

Analysis of intraspecies polymorphism in the ribosomal DNA cluster of the cockroach Blattella germanica.

作者信息

Mukha D V, Sidorenko A P, Lazebnaya I V, Wiegmann B M, Schal C

机构信息

Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia.

出版信息

Insect Mol Biol. 2000 Apr;9(2):217-22. doi: 10.1046/j.1365-2583.2000.00175.x.

DOI:10.1046/j.1365-2583.2000.00175.x

PMID:10762430

Abstract

HindIII restriction digests of the rDNA repeat unit of the German cockroach, Blattella germanica, reveal significant intraspecies sequence polymorphism. This variability is probably caused by structural differences within the nontranscribed spacer regions (NTS) of the ribosomal repeat unit. HindIII rDNA fragment polymorphisms in three cockroach strains show that individuals from different populations may have different HindIII rDNA patterns, whereas individuals within populations exhibit relatively similar rDNA patterns. We suggest that HindIII restriction fragment polymorphisms within cockroach ribosomal DNA will be a valuable tool for measuring population-level parameters within and between natural cockroach populations.

摘要

德国小蠊核糖体DNA重复单元的HindIII酶切显示出显著的种内序列多态性。这种变异性可能是由核糖体重复单元的非转录间隔区（NTS）内的结构差异引起的。三种蟑螂品系的HindIII rDNA片段多态性表明，来自不同种群的个体可能具有不同的HindIII rDNA模式，而种群内的个体表现出相对相似的rDNA模式。我们认为，蟑螂核糖体DNA内的HindIII限制性片段多态性将是测量自然蟑螂种群内部和之间种群水平参数的一个有价值的工具。