Hashemi-Aghdam Saedeh Sadat, Rafie Golnaz, Akbari Sanaz, Oshaghi Mohammad Ali
Deptartment of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
J Arthropod Borne Dis. 2017 May 27;11(2):182-193. eCollection 2017 Jun.
Cockroaches are of vital importance medically and hygienically as they can disperse human pathogenic agents and are especially responsible for food contamination and spreading of food borne pathogens. In this study, part of mtDNA-COI gene of five common pest cockroaches was tested for diagnostic and phylogenetic purposes.
We have described barcode region of mtDNA-COI gene of five cockroach species: , and along with the development of a PCR-RFLP method for rapid detection and differentiation of these health pest species.
The PCR generates a single 710 bp-sized amplicon in all cockroach specimens, followed by direct sequencing. I predicted from the sequencing data provided different RFLP profiles among five species. There was a significant intra-species variation within the American cockroach populations, but no genetic variation within other species. Accordingly, phylogenetic analysis demonstrates common monophyly for cockroach families in agreement with conventional taxonomy. However (Ectobiidae) diverged as an early ancestor of other cockroaches and was not associated with other Ectobiidae.
The PCR-RFLP protocol might be useful when the conventional taxonomic methods are not able to identify specimens, particularly when only small body parts of specimens are available or they are in a decaying condition. mtDNA-COI gene shows potentially useful for studying phylogenetic relationships of Blattodea order.
蟑螂在医学和卫生方面至关重要，因为它们能传播人类病原体，尤其在食物污染和食源性病原体传播方面负有责任。在本研究中，对五种常见害虫蟑螂的线粒体DNA细胞色素氧化酶亚基I（mtDNA-COI）基因的部分片段进行检测，用于诊断和系统发育研究。
我们描述了五种蟑螂物种的mtDNA-COI基因条形码区域，以及一种用于快速检测和区分这些有害健康物种的聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法。
PCR在所有蟑螂标本中产生一个单一的710 bp大小的扩增子，随后进行直接测序。根据测序数据预测，五种物种之间存在不同的RFLP图谱。美国蟑螂种群内存在显著的种内变异，但其他物种内没有遗传变异。因此，系统发育分析表明蟑螂科具有共同的单系性，与传统分类法一致。然而，姬蠊科（Ectobiidae）作为其他蟑螂的早期祖先分化出来，且与其他姬蠊科没有关联。
当传统分类方法无法鉴定标本时，特别是当只有标本的小身体部分可用或它们处于腐烂状态时，PCR-RFLP方案可能会有用。mtDNA-COI基因显示出对研究蜚蠊目系统发育关系有潜在用途。
