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[在含有蓝藻的培养基中鱼组织的氨基酸组成和转氨酶活性]

[Amino acid composition and transaminase activity in fish tissues, in a medium containing Cyanophyceae].

作者信息

Maliarevskaia A Ia, Birger T I, Solomatina V D, Gupalo Iu M

出版信息

Ukr Biokhim Zh (1978). 1978 Nov-Dec;50(6):718-22.

PMID:107639
Abstract

The content of free amino acids, activity of aspartate and alanine transaminase, number of sulphydryl groups in fish tissues were studied as affected by lethal amounts (3.2 g/l) of blue-green algae. Blue-green algae have a certain affect on fishes not only by excreting biologically active substances in the process of vital activity and decay but also changing the gas regime of the medium (the oxygen content lowers, the amount of carbon dioxide increases). Under the algae effect the total content of free amino acids in the fish liver, intestine and muscles increases, mainly due to a rise in the content of glutamic acid with threonine and aspartic acid with serine. These changes are most essential in the liver, intestine and are less pronounced in the muscles. Under the effect of blue-green algae the activity of aspartate transaminase increases in the heart, brain and decreases in the intestine. The activity of alanine transaminase enhances in the heart, intestine and brain. The ration value for these enzymes changes significantly in the brain, liver, intestine, but does not differ from the control in the muscles.

摘要

研究了致死量(3.2克/升)蓝藻对鱼组织中游离氨基酸含量、天冬氨酸转氨酶和丙氨酸转氨酶活性以及巯基数量的影响。蓝藻对鱼类产生一定影响,不仅在其生命活动和腐烂过程中分泌生物活性物质,还改变介质的气体状态(氧气含量降低,二氧化碳含量增加)。在蓝藻的作用下,鱼肝、肠和肌肉中游离氨基酸的总含量增加,主要是由于谷氨酸与苏氨酸以及天冬氨酸与丝氨酸含量的升高。这些变化在肝脏和肠道中最为显著,在肌肉中则不太明显。在蓝藻的作用下,心脏和大脑中天冬氨酸转氨酶的活性增加,而肠道中则降低。丙氨酸转氨酶的活性在心脏、肠道和大脑中增强。这些酶在大脑、肝脏、肠道中的比值变化显著,但在肌肉中与对照组无差异。

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