哺乳动物降钙素受体样受体/受体活性修饰蛋白复合物在果蝇施奈德2细胞中定义了降钙素基因相关肽和肾上腺髓质素受体。

Mammalian calcitonin receptor-like receptor/receptor activity modifying protein complexes define calcitonin gene-related peptide and adrenomedullin receptors in Drosophila Schneider 2 cells.

作者信息

Aldecoa A, Gujer R, Fischer J A, Born W

机构信息

Research Laboratory for Calcium Metabolism, Departments of Orthopaedic Surgery and Medicine, University of Zurich, Klinik Balgrist, Forchstrasse 340, 8008, Zurich, Switzerland.

出版信息

FEBS Lett. 2000 Apr 14;471(2-3):156-60. doi: 10.1016/s0014-5793(00)01387-9.

DOI:10.1016/s0014-5793(00)01387-9

PMID:10767413

Abstract

Differential glycosylation of human and rat (r) calcitonin (CT) receptor-like receptors (CRLR) as a result of interactions with accessory receptor activity-modifying proteins (RAMP)1 or -2 was considered to define CT gene-related peptide (CGRP) or adrenomedullin (ADM) receptors in mammalian cells. Here, Drosophila Schneider (S2) cells stably co-expressed rCRLR and RAMP1 or -2 as functional CGRP or ADM receptors. Different from mammalian cells, rCRLR expressed in S2 cells are uniformly glycosylated proteins independent of RAMP1 or RAMP2. Bis(sulfosuccinimidyl)suberate cross-linking revealed receptor components with the size of rCRLR, increased by the molecular weights of the corresponding RAMPs and [(125)I]CGRP or [(125)I]ADM. In conclusion, [(125)I]CGRP/rCRLR/RAMP1 and [(125)I]ADM/rCRLR/RAMP2 complexes have been recognized in Drosophila S2 cells.

摘要

由于与辅助受体活性调节蛋白（RAMP）1或 -2相互作用导致的人和大鼠（r）降钙素（CT）受体样受体（CRLR）的差异糖基化，被认为可在哺乳动物细胞中定义降钙素基因相关肽（CGRP）或肾上腺髓质素（ADM）受体。在此，果蝇施奈德（S2）细胞稳定共表达rCRLR和RAMP1或 -2作为功能性CGRP或ADM受体。与哺乳动物细胞不同，S2细胞中表达的rCRLR是均匀糖基化的蛋白质，与RAMP1或RAMP2无关。双（磺基琥珀酰亚胺）辛二酸酯交联显示受体成分的大小与rCRLR相同，并因相应RAMP和[（125）I]CGRP或[（125）I]ADM的分子量而增加。总之，在果蝇S2细胞中已识别出[（125）I]CGRP/rCRLR/RAMP1和[（125）I]ADM/rCRLR/RAMP2复合物。