Kusakabe T, Maeda M, Hoshi N, Sugino T, Watanabe K, Fukuda T, Suzuki T
Department of Pathology, Fukushima Medical University School of Medicine, Fukushima, Japan.
J Histochem Cytochem. 2000 May;48(5):613-22. doi: 10.1177/002215540004800505.
Animal fatty acid synthase (FAS) is a homodimer protein which synthesizes long-chain fatty acids and is rich in liver, brain, breast, and lung. However, the precise cellular localization of FAS in human tissues has not been elucidated. Immunohistochemistry with a new antibody to human FAS revealed that in adult human tissues FAS is distributed mainly in cells with high lipid metabolism (adipocytes, corpus luteum, hepatocytes, sebaceous glands, and Type II alveolar cells), in hormone-sensitive cells (anterior pituitary, apocrine gland, breast, endometrium, prostate, seminal vesicle, and adrenal cortex), and in a subset of epithelial cells of duodenum and stomach, colon absorptive cells, cerebral neurons, basket cells of cerebellum, decidua, uroepithelium, and epidymis. In fetal cells at 20 weeks of gestation, FAS was mainly present in proliferative epithelial cells of the digestive and respiratory systems, proximal renal tubules, adrenocortical cells, and mesenchymal and hematolymphoid cells. Staining was significant in nonproliferating cells, as observed in adult, and in sympathetic ganglion cells, Leidig cells of testis, and Langhans cells of chorionic villi. FAS is maintained in hormone-sensitive cells and/or cells active in lipid metabolism in the adult and is expressed in proliferating cells in the fetus, suggesting active fatty acid synthesis for energy utilization or membrane lipids.
动物脂肪酸合酶（FAS）是一种同二聚体蛋白，可合成长链脂肪酸，在肝脏、大脑、乳腺和肺中含量丰富。然而，FAS在人体组织中的精确细胞定位尚未阐明。使用一种针对人FAS的新抗体进行免疫组织化学分析显示，在成人组织中，FAS主要分布于脂质代谢活跃的细胞（脂肪细胞、黄体、肝细胞、皮脂腺和Ⅱ型肺泡细胞）、激素敏感细胞（垂体前叶、顶泌汗腺、乳腺、子宫内膜、前列腺、精囊和肾上腺皮质）以及十二指肠和胃的一部分上皮细胞、结肠吸收细胞、脑神经元、小脑篮状细胞、蜕膜、尿路上皮和附睾中。在妊娠20周的胎儿细胞中，FAS主要存在于消化和呼吸系统的增殖上皮细胞、近端肾小管、肾上腺皮质细胞以及间充质和血液淋巴样细胞中。与成人一样，在非增殖细胞以及交感神经节细胞、睾丸间质细胞和绒毛膜朗汉斯细胞中染色明显。FAS在成人的激素敏感细胞和/或脂质代谢活跃的细胞中维持表达，在胎儿的增殖细胞中表达，提示脂肪酸合成活跃以用于能量利用或膜脂合成。
