用于通过聚合酶链反应筛选酵母克隆的DNA提取方法。
DNA extraction method for screening yeast clones by PCR.
作者信息
Akada R, Murakane T, Nishizawa Y
机构信息
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Ube, Japan.
出版信息
Biotechniques. 2000 Apr;28(4):668-70, 672, 674. doi: 10.2144/00284st02.
A simple procedure for isolating yeast DNA suitable for use as a template for PCR amplification is described. SDS treatment alone is sufficient for extraction of chromosomal DNA from yeast cells. Cells of a yeast colony are suspended in a small volume (about 20 microL) of a 0.25% SDS solution, mixed vigorously and centrifuged. The supernatant can be directly used as a template after dilution to give an SDS concentration of less than 0.01% in the final PCR mixture.
本文描述了一种简单的用于分离适合作为PCR扩增模板的酵母DNA的方法。单独使用SDS处理就足以从酵母细胞中提取染色体DNA。将酵母菌落的细胞悬浮于少量（约20微升）0.25%的SDS溶液中，剧烈混合并离心。上清液在稀释后可直接用作模板，以使最终PCR混合物中的SDS浓度低于0.01%。
Zotero 插件