Gabbert H, Wagner R, Aust P, Höhn P
Acta Histochem. 1978;63(2):197-213. doi: 10.1016/S0065-1281(78)80026-9.
After ligation of the vascular arcades of the upper jejunum in rats, the ischemic damage to the intestinal mucosa and its regenerative behavior after ischemia lasting 120 minutes were investigated with histological and enzyme-histochemical methods. During the ischemic injury of the jejunal mucosa, there is rejection of hydropically swollen epithelial cells into the intestinal lumen advancing from the tip to the base of the villi without a previously detectable loss of activity of the enzymes investigated. At the end of ischemia lasting 120 minutes, there is complete destruction of the villi as well as the upper portions of the crypts. After rapid re-epithelialization of the mucosal surface by a flat epithelium, reformation of villi begins already after a 24 h period of regeneration. On the third day of regeneration, the intestinal mucosa has plump villi again. Their epithelia already show the morphological characteristics of mature enterocytes. On the other hand, enzymatic maturation of the enterocytes is only concluded between the first and second week of regeneration. An altered proliferation kinetics of the crypt epithelia due to the repair process is discussed as cause of this delayed enzymatic maturation of the enterocytes relative to the morphological differentiation. Although the intestinal mucosa has a regular villous structure again after regeneration lasting 8 days, enzyme histochemical finding in the lamina propria indicate that the process of repair is only complete here in the sixth week of regeneration.
在结扎大鼠空肠上段的血管弓后，采用组织学和酶组织化学方法研究了肠黏膜的缺血性损伤及其在持续120分钟缺血后的再生行为。在空肠黏膜缺血损伤期间，从绒毛顶端向基部推进的水肿肿胀上皮细胞被排入肠腔，而所研究的酶活性此前并未出现可检测到的丧失。在持续120分钟的缺血结束时，绒毛以及隐窝上部完全被破坏。在黏膜表面由扁平上皮快速重新上皮化后，在再生24小时后就已开始绒毛的重新形成。在再生的第三天，肠黏膜再次出现丰满的绒毛。其上皮已经显示出成熟肠上皮细胞的形态特征。另一方面，肠上皮细胞的酶成熟仅在再生的第一周和第二周之间完成。由于修复过程导致隐窝上皮细胞增殖动力学改变，被认为是肠上皮细胞酶成熟相对于形态分化延迟的原因。尽管在持续8天的再生后肠黏膜再次具有规则的绒毛结构，但固有层中的酶组织化学结果表明，此处的修复过程仅在再生的第六周完成。
