从多发性硬化症患者中纯化的α2-巨球蛋白稳定性降低。
Decreased stability of alpha2-macroglobulin purified from patients with multiple sclerosis.
作者信息
Gunnarsson M, Stigbrand T, Jensen P E
机构信息
Department of Immunology, Umeå University, Sweden.
出版信息
Acta Neurol Scand. 2000 Apr;101(4):270-2. doi: 10.1034/j.1600-0404.2000.101004270.x.
OBJECTIVES
To investigate conformational properties of alpha2-macroglobulin from multiple sclerosis patients.
MATERIALS AND METHODS
alpha2-macroglobulin was purified to homogeneity from plasma of 4 multiple sclerosis patients and 5 healthy controls. The plasma and the purified alpha2-macroglobulin from each individual were investigated using polyacrylamide gel electrophoresis.
RESULTS
Impaired stability of purified alpha2-macroglobulins from multiple sclerosis patients was demonstrated with spontaneous conversion to an electrophoretic "fast" form upon purification and following storage not ascribable to bait region cleavage.
CONCLUSION
alpha2-macroglobulin from multiple sclerosis patients displays altered stability. Possible functional impairments of proteinase inhibition mechanisms are discussed.
目的
研究多发性硬化症患者α2-巨球蛋白的构象特性。
材料与方法
从4例多发性硬化症患者和5例健康对照者的血浆中纯化α2-巨球蛋白至均一性。使用聚丙烯酰胺凝胶电泳对每个个体的血浆和纯化的α2-巨球蛋白进行研究。
结果
多发性硬化症患者纯化的α2-巨球蛋白稳定性受损，纯化后以及储存后会自发转化为电泳“快”型，这并非由于诱饵区裂解所致。
结论
多发性硬化症患者的α2-巨球蛋白显示出稳定性改变。讨论了蛋白酶抑制机制可能存在的功能损害。
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