Akyön Y, Ergüven S, Arikan S, Yurdakök K, Günalp A
Department of Microbiology and Clinical Microbiology, Hacettepe University Faculty of Medicine, Ankara.
Turk J Pediatr. 1999 Apr-Jun;41(2):189-96.
Stool samples from two hundred children with diarrhea and from 50 healthy children were examined, by modified Kinyoun's acid-fast staining (MAF), Giemsa staining and direct (DFA) and indirect immunofluorescence antibody (IFA) methods, in order to determine cryptosporidiosis prevalence under the age of 12 and to detect the most efficient identifying method for use in our country. Cryptosporidium oocysts were detected in seven (3.5%) of the cases. None of the samples from the control subjects was found to be positive for Cryptosporidium. Our results indicate that Cryptosporidial oocysts should be detected in children with diarrhea. Modified Kinyoun staining method is practical and reliable for this purpose. Immunofluorescence staining methods can be applied for conformation of the results, if available.
为确定12岁以下儿童隐孢子虫病的患病率，并找出我国最有效的检测方法，采用改良金胺酚染色法（MAF）、吉姆萨染色法、直接免疫荧光抗体法（DFA）和间接免疫荧光抗体法（IFA），对200例腹泻儿童和50例健康儿童的粪便样本进行了检测。在7例（3.5%）病例中检测到隐孢子虫卵囊。对照组样本中未发现隐孢子虫阳性。我们的结果表明，腹泻儿童应检测隐孢子虫卵囊。改良金胺酚染色法在此目的上实用且可靠。如有条件，免疫荧光染色法可用于结果的确认。
