Roth A L, Shoop R D, Berg D K
Department of Biology, 0357, University of California-San Diego, La Jolla, CA 92093, USA.
Eur J Pharmacol. 2000 Mar 30;393(1-3):105-12. doi: 10.1016/s0014-2999(00)00092-3.
Nicotinic receptors containing the alpha7 gene product are widely expressed in the nervous system and have a high relative permeability to Ca(2+). This permits them to influence a variety of Ca(2+)-dependent events in neurons. On chick ciliary ganglion neurons, the receptors are concentrated on somatic spines and contribute directly to postsynaptic signaling. Receptors containing the alpha7 gene product can also be found in the chick sciatic nerve being transported to distal locations. Both motoneurons and dorsal root ganglion neurons are candidate sources of the receptors since both extend processes into the nerve and synthesize alpha7 protein. Immunoprecipitation assays with subunit-specific monoclonal antibodies and pharmacological comparisons fail to detect differences between sciatic nerve and ciliary ganglion alpha7-containing receptors. Cell-specific machinery and receptor posttranslational modifications may determine which sites the receptors populate.
含有α7基因产物的烟碱型受体在神经系统中广泛表达，对Ca(2+)具有较高的相对通透性。这使得它们能够影响神经元中各种依赖Ca(2+)的事件。在鸡睫状神经节神经元上，这些受体集中在体细胞棘突上，并直接参与突触后信号传递。含有α7基因产物的受体也可在向远端运输的鸡坐骨神经中发现。运动神经元和背根神经节神经元都是这些受体的候选来源，因为它们都向神经中延伸突起并合成α7蛋白。用亚基特异性单克隆抗体进行的免疫沉淀试验和药理学比较未能检测到坐骨神经和睫状神经节含α7受体之间的差异。细胞特异性机制和受体翻译后修饰可能决定了受体在哪些位点聚集。
