González S, Navarro M, López H, López M C, Pérez V
University of Granada, Faculty of Pharmacy, Department of Nutrition and Bromatology, Spain.
J AOAC Int. 2000 Mar-Apr;83(2):377-81.
An efficient method was developed for the determination of nanogram levels of lithium in biological samples. Serum samples from human subjects from southeastern Spain, treated or not treated with lithium carbonate, were analyzed by electrothermal atomic absorption spectrometry. The samples were previously treated with a matrix modifier consisting of 0.1% Triton X-100 and injected through a graphite tube with L'vov platform. The Li concentrations measured by the procedure described for the 3 certified reference samples used were not significantly different (p > 0.05) than certified levels. Sample recoveries and variability during several days, with coefficients of variation from 4.00 to 14.8%, demonstrated the reliability and accuracy of this technique. Mean Li concentration determined in the serum of individuals with psychiatric disorders treated with Li (n = 117, 5.077 +/- 1.795 microg Li/mL) was significantly higher (p < 0.001) than that in individuals not treated with Li (n = 24, 1.902 +/- 2.054 ng Li/mL).
开发了一种测定生物样品中纳克级锂含量的有效方法。对来自西班牙东南部的人体受试者的血清样本进行分析，这些受试者使用或未使用碳酸锂进行治疗，采用电热原子吸收光谱法进行检测。样本预先用由0.1% Triton X - 100组成的基体改进剂处理，并通过带有L'vov平台的石墨管进样。通过所述方法测定的3种认证参考样品的锂浓度与认证水平无显著差异（p > 0.05）。样本回收率以及几天内的变异性，变异系数在4.00%至14.8%之间，证明了该技术的可靠性和准确性。接受锂治疗的精神疾病患者血清中锂的平均浓度（n = 117，5.077 +/- 1.795微克锂/毫升）显著高于未接受锂治疗的个体（n = 24，1.902 +/- 2.054纳克锂/毫升）（p < 0.001）。
