Gardiner P H, Littlejohn D, Halls D J, Fell G S
Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, UK.
J Trace Elem Med Biol. 1995 Jul;9(2):74-81. doi: 10.1016/S0946-672X(11)80014-3.
A method is described for the direct determination of selenium in serum or plasma by electrothermal atomic absorption spectrometry with deuterium-arc background correction. Samples are diluted (1 + 2) with a modifier containing palladium nitrate and Triton X-100. Samples are atomised from a L'vov platform in a pyrolytically-coated electrographite tube and peak area signals are measured. Direct determination is possible by using selenium standards matched to the physiological concentrations of sodium chloride, calcium and phosphate. The detection limit is 6 micrograms/L in the original sample. Precision at a selenium concentration of 97 micrograms/L was 2.2% RSD within batch and 3.0% RSD between batch. Accuracy is shown by (i) analysis of a Seronorm reference serum (value obtained 97 +/- 3 micrograms/L; recommended value 96 micrograms/L); (ii) recovery of added selenium (93.3 +/- 6.7% and 98.2 +/- 3.3% at additions of 30 and 60 micrograms/L, respectively) and (iii) comparison of results with mean of all laboratories in an external quality assessment scheme.
本文描述了一种采用氘弧背景校正的电热原子吸收光谱法直接测定血清或血浆中硒的方法。样品用含有硝酸钯和吐温X - 100的改性剂按（1 + 2）进行稀释。样品在热解涂层石墨管中的L'vov平台上进行原子化，并测量峰面积信号。通过使用与氯化钠、钙和磷酸盐生理浓度匹配的硒标准品可进行直接测定。原始样品中的检测限为6微克/升。在硒浓度为97微克/升时，批内精密度为2.2%相对标准偏差，批间精密度为3.0%相对标准偏差。通过以下方式表明准确性：（i）分析Seronorm参考血清（获得的值为97±3微克/升；推荐值为96微克/升）；（ii）添加硒的回收率（分别在添加30和60微克/升时为93.3±6.7%和98.2±3.3%）以及（iii）在外部质量评估计划中将结果与所有实验室的平均值进行比较。
