Sirota L, Punsky I, Bessler H
Neonatal Intensive Care Unit, Schneider Children's Medical Center of Israel.
Acta Paediatr. 2000 Mar;89(3):331-5.
The in vitro effect of indomethacin (IM) on the production of interleukin (IL)-1beta, IL-6 and tumor necrosis factor (TNF)alpha by cord blood mononuclear cells (CBMC) from preterm newborns was compared to that of peripheral blood MC of adults (PBMC). MC isolated from peripheral blood of adults (PBMC) and cord blood of preterm newborns (CBMC) were incubated without or with lipopolysaccharide (LPS) in the absence or presence of various concentrations of IM. The levels of IL-1beta, IL-6 and TNFalpha in the supernatants were tested by ELISA. MC isolated from preterm newborns were less sensitive to the in vitro effect of IM on IL-1beta and TNFalpha secretion than adult cells. While the spontaneous secretion of IL-1beta and TNFalpha and the production of TNFalpha induced by LPS were significantly increased following incubation of adult PBMC with IM, only the spontaneous synthesis of TNFalpha by CBMC of preterm newborns was affected by this drug. The in vitro production of IL-6 by MC in the two groups was not affected by the addition of IM. It is suggested that IM may affect the preterm's immune response. However, the role of the drug in the frequency and severity of infections in the neonatal intensive care unit needs further investigation.
将吲哚美辛（IM）对早产新生儿脐带血单个核细胞（CBMC）产生白细胞介素（IL）-1β、IL-6和肿瘤坏死因子（TNF）α的体外作用，与成人外周血单个核细胞（PBMC）的作用进行了比较。从成人外周血（PBMC）和早产新生儿脐带血（CBMC）中分离出的单个核细胞，在无脂多糖（LPS）或有脂多糖的情况下，于不同浓度的IM存在或不存在时进行孵育。通过酶联免疫吸附测定法（ELISA）检测上清液中IL-1β、IL-6和TNFα的水平。与成人细胞相比，从早产新生儿分离出 的单个核细胞对IM体外影响IL-1β和TNFα分泌的作用较不敏感。当成人PBMC与IM孵育后，IL-1β和TNFα的自发分泌以及LPS诱导的TNFα产生显著增加，而IM仅影响早产新生儿CBMC自发合成TNFα。两组单个核细胞体外产生IL-6不受添加IM的影响。提示IM可能影响早产新生儿的免疫反应。然而，该药物在新生儿重症监护病房感染的发生率和严重程度方面的作用需要进一步研究。
