Morgan J A, Barney C S, Penn A H, Shanks J V
Department of Chemical Engineering, Iowa State University, Ames 50011-2230, USA.
Appl Microbiol Biotechnol. 2000 Mar;53(3):262-5. doi: 10.1007/s002530050018.
The influence of buffered media upon the growth and alkaloid productivity of Catharanthus roseus hairy root culture was examined. As expected, the buffers minimized shifts in the pH of the media and had slightly negative effects upon growth. The growth of the hairy roots remained optimal in unbuffered media. The specific yield of lochnericine was significantly lower in response to the addition of buffers, while tabersonine was significantly higher. In contrast, the specific yields of ajmalicine, serpentine, and horhammericine remained unchanged.
研究了缓冲介质对长春花毛状根培养物生长和生物碱产量的影响。正如预期的那样，缓冲剂使培养基的pH值变化最小化，并且对生长有轻微的负面影响。在未缓冲的培养基中，毛状根的生长保持最佳状态。添加缓冲剂后，洛柯辛碱的比产量显著降低，而利血平碱则显著升高。相比之下，阿吗碱、蛇根碱和霍哈默辛碱的比产量保持不变。
