Shirako Y, Suzuki N, French R C
Asian Center for Bioresources and Environmental Sciences (ANESC), University of Tokyo, Bunkyo-ku, Tokyo, 113-8657, Japan.
Virology. 2000 Apr 25;270(1):201-7. doi: 10.1006/viro.2000.0251.
Nucleotide sequences of RNAs 1 and 2 of a Japanese strain of soil-borne wheat mosaic virus (SBWMV), the type species of the genus Furovirus, and sorghum chlorotic spot virus (SCSV) were determined from cloned cDNA. The relationship among the Japanese and US strains of SBWMV, SCSV, oat golden stripe virus (OGSV), and recently proposed Chinese wheat mosaic and European wheat mosaic viruses (CWMV and EWMV) were examined at the nucleotide and amino acid levels. Pairwise comparisons of genome-encoded proteins among the six viruses showed that the US strains of SBWMV and CWMV were the most closely related pair in RNA 1 and the Japanese strains of SBWMV and EWMV were most closely related in RNA 2. SCSV was most distantly related to the other five viruses. Phylogenetic analysis indicated that there may have been an ancient reassortment between RNAs 1 and 2 of the four wheat-infecting viruses and OGSV, while SCSV was shown to have separated from the rest before the other five viruses diverged. The fact that CWMV and EWMV have almost identical biological properties as well as the sequence similarities to the two strains of SBWMV suggests that they be regarded as strains of SBWMV, considering that SBWMV consists of genetically diverged strains. OGSV and SCSV are distinct in biological properties in addition to genetic divergence in the genus Furovirus.
从克隆的cDNA中测定了土传小麦花叶病毒（SBWMV）日本株系（Furovirus属的模式种）以及高粱褪绿斑病毒（SCSV）的RNA 1和RNA 2的核苷酸序列。在核苷酸和氨基酸水平上研究了SBWMV的日本和美国株系、SCSV、燕麦金色条纹病毒（OGSV）以及最近提出的中国小麦花叶病毒和欧洲小麦花叶病毒（CWMV和EWMV）之间的关系。六种病毒之间基因组编码蛋白的成对比较表明，SBWMV的美国株系和CWMV在RNA 1中关系最密切，而SBWMV的日本株系和EWMV在RNA 2中关系最密切。SCSV与其他五种病毒的关系最远。系统发育分析表明，四种感染小麦的病毒与OGSV的RNA 1和RNA 2之间可能存在古老的重配，而SCSV在其他五种病毒分化之前就已与其余病毒分离。鉴于CWMV和EWMV具有几乎相同的生物学特性以及与SBWMV的两个株系的序列相似性，考虑到SBWMV由基因分化的株系组成，建议将它们视为SBWMV的株系。除了Furovirus属中的遗传差异外，OGSV和SCSV在生物学特性上也不同。
