Appaix F, Minatchy M, Riva-Lavieille C, Olivares J, Antonsson B, Saks V A
Laboratory of Bioenergetics, Joseph Fourier University, P.O. Box 53X, 38041, Grenoble, France.
Biochim Biophys Acta. 2000 Apr 21;1457(3):175-81. doi: 10.1016/s0005-2728(00)00098-0.
This paper recalls the earlier work by Keilin, Margoliash and others at the beginning of the 20th century and shows how their results can be used for the rapid solution of new problems of modern science. It describes a rapid and simple spectrophotometric method for quantitative determination of cytochrome c release from isolated mitochondria or permeabilized cells induced by proapoptotic proteins. For this, the Soret (gamma) peak at 414 nm in the spectrum of cytochrome c is used. The results of spectrophotometric assay of cytochrome c release are in accord with those of oxygraphic determination of cytochrome c-dependent respiration of isolated mitochondria and permeabilized cardiomyocytes.
本文回顾了20世纪初凯林、马戈利亚什等人的早期工作，并展示了如何将他们的研究成果用于快速解决现代科学中的新问题。它描述了一种快速简便的分光光度法，用于定量测定由促凋亡蛋白诱导的分离线粒体或透化细胞中细胞色素c的释放。为此，利用了细胞色素c光谱中414nm处的索雷特（γ）峰。细胞色素c释放的分光光度测定结果与分离线粒体和透化心肌细胞的细胞色素c依赖性呼吸的氧电极测定结果一致。
