Wu Y, Parent A
Laboratoire de Neurobiologie, Centre de Recherche Université Laval Robert-Giffard, 2601 Chemin de la Canardière, Local F-6500, Beauport, Canada.
Brain Res. 2000 Apr 28;863(1-2):182-91. doi: 10.1016/s0006-8993(00)02135-1.
The present study is aimed at evaluating the relative number and comparing the pattern of distribution of interneurons containing calretinin (CR), parvalbumin (PV) or NADPH-d in the striatum of rats, squirrel monkeys and humans. A series of adjacent coronal sections taken at three comparable rostrocaudal levels in the three species were treated to reveal the three neuronal markers and the density of each type of chemospecific interneurons was analyzed with a computerized image analysis system. In primates, the most abundant interneurons were those expressing CR. The ratio of CR+/PV+ neurons was approximately 2-3:1 compared to a ratio of 3-4:1 for CR+/NADPH-d+ neurons. In contrast, the most frequently encountered interneurons in the rat striatum were those expressing PV. In rodents, all three interneurons were more abundant rostrally than caudally, but CR+ neurons displayed a particularly striking rostrocaudal decreasing gradient. In monkeys and humans, the three striatal interneurons were distributed rather uniformly rostrocaudally, but CR+ and PV+ interneurons were significantly more numerous in the caudate nucleus than in the putamen in humans. In monkeys, only PV+ neurons were more abundant in the caudate nucleus than in putamen. Overall, the density of the three striatal interneurons was much higher in monkeys than in rats and humans. These results reveal important species differences in respect to the relative density and pattern of distribution of striatal interneurons. These findings should be taken into account when evaluating the effect of neurodegenerative processes on cell densities in the human striatum or when studying animal models of the such diseases.
本研究旨在评估含钙视网膜蛋白（CR）、小白蛋白（PV）或还原型辅酶Ⅱ黄递酶（NADPH-d）的中间神经元在大鼠、松鼠猴和人类纹状体中的相对数量，并比较其分布模式。在这三个物种中，于三个可比的前后水平获取一系列相邻冠状切片，对其进行处理以显示这三种神经元标志物，并使用计算机图像分析系统分析每种化学特异性中间神经元的密度。在灵长类动物中，最丰富的中间神经元是那些表达CR的神经元。CR+/PV+神经元的比例约为2-3:1，而CR+/NADPH-d+神经元的比例为3-4:1。相比之下，大鼠纹状体中最常见的中间神经元是那些表达PV的神经元。在啮齿动物中，所有三种中间神经元在前部比后部更丰富，但CR+神经元显示出特别明显的前后递减梯度。在猴子和人类中，三种纹状体中间神经元在前后方向上分布较为均匀，但在人类中，CR+和PV+中间神经元在尾状核中的数量明显多于壳核。在猴子中，只有PV+神经元在尾状核中的数量比壳核中更多。总体而言，猴子纹状体中三种中间神经元的密度远高于大鼠和人类。这些结果揭示了纹状体中间神经元在相对密度和分布模式方面的重要物种差异。在评估神经退行性过程对人类纹状体细胞密度的影响或研究此类疾病的动物模型时，应考虑这些发现。
