一种新型仓鼠模型中1型马疱疹病毒灭活疫苗的效力

Efficacy of an inactivated vaccine for equine herpesvirus type 1 in a novel hamster model.

作者信息

Skinner G R, Davies J

机构信息

Department of Infection, University of Birmingham, Birmingham, UK.

出版信息

Intervirology. 2000;43(1):27-35. doi: 10.1159/000025020.

DOI:10.1159/000025020

PMID:10773735

Abstract

A vaccine designated F.EHV1(S(-))BHK, which was prepared by formaldehyde treatment of equine herpesvirus type 1 (EHV1)-infected baby hamster kidney cells, stimulated neutralising antibodies in hamsters and rabbits and protected new-born hamsters against a lethal challenge with EHV1 by vaccination of their pregnant mothers. The preparative method was then modified to eliminate virus particles and intraparticulate DNA, producing a vaccine designated Bg.F.EHV1(S(-))BHK. this modification stimulated neutralising antibodies with evidence of in vivo inactivation of the virus above non-specific levels in a mouse model. There were no adverse effects from these vaccines in rodent or rabbit species.

摘要

一种名为F.EHV1(S(-))BHK的疫苗，是通过用甲醛处理感染了1型马疱疹病毒（EHV1）的幼仓鼠肾细胞制备而成的，它能刺激仓鼠和兔子产生中和抗体，并通过给怀孕母鼠接种疫苗，保护新生仓鼠免受EHV1的致命攻击。随后对制备方法进行了改进，以去除病毒颗粒和颗粒内的DNA，生产出一种名为Bg.F.EHV1(S(-))BHK的疫苗。这种改进刺激产生了中和抗体，在小鼠模型中，有证据表明病毒在体内的灭活程度高于非特异性水平。这些疫苗对啮齿动物或兔子没有不良影响。