Poch G K, Klette K L, Anderson C
Navy Drug Screening Laboratory, San Diego, California 92134-7040, USA.
J Anal Toxicol. 2000 Apr;24(3):170-9. doi: 10.1093/jat/24.3.170.
This paper compares the potential forensic application of two sensitive and rapid procedures (liquid chromatography-mass spectrometry and liquid chromatography-ion trap mass spectrometry) for the detection and quantitation of 2-oxo-3-hydroxy lysergic acid diethylamide (O-H-LSD) a major LSD metabolite. O-H-LSD calibration curves for both procedures were linear over the concentration range 0-8,000 pg/mL with correlation coefficients (r2) greater than 0.99. The observed limit of detection (LOD) and limit of quantitation (LOQ) for O-H-LSD in both procedures was 400 pg/mL. Sixty-eight human urine specimens that had previously been found to contain LSD by gas chromatography-mass spectrometry were reanalyzed by both procedures for LSD and O-H-LSD. These specimens contained a mean concentration of O-H-LSD approximately 16 times higher than the LSD concentration. Because both LC methods produce similar results, either procedure can be readily adapted to O-H-LSD analysis for use in high-volume drug-testing laboratories. In addition, the possibility of significantly increasing the LSD detection time window by targeting this major LSD metabolite for analysis may influence other drug-free workplace programs to test for LSD.
本文比较了两种灵敏快速的方法（液相色谱 - 质谱联用和液相色谱 - 离子阱质谱联用）在检测和定量2-氧代-3-羟基麦角酰二乙胺（O-H-LSD，一种主要的麦角酰二乙胺代谢物）方面的潜在法医应用。两种方法的O-H-LSD校准曲线在0 - 8000 pg/mL的浓度范围内呈线性，相关系数（r2）大于0.99。两种方法中O-H-LSD的检测限（LOD）和定量限（LOQ）均为400 pg/mL。通过气相色谱 - 质谱联用先前已发现含有麦角酰二乙胺的68份人类尿液标本，采用这两种方法重新分析其中的麦角酰二乙胺和O-H-LSD。这些标本中O-H-LSD的平均浓度比麦角酰二乙胺浓度高约16倍。由于两种液相色谱方法产生相似的结果，任何一种方法都可轻松适用于O-H-LSD分析，以供大量药物检测实验室使用。此外，通过针对这种主要的麦角酰二乙胺代谢物进行分析，显著延长麦角酰二乙胺检测时间窗口的可能性，可能会影响其他无毒品工作场所计划对麦角酰二乙胺进行检测。
