Mottier P, Parisod V, Turesky R J
Nestlé Research Center, Nestec Ltd., Vers-chez-les-Blanc, 1000 Lausanne 26, Switzerland.
J Agric Food Chem. 2000 Apr;48(4):1160-6. doi: 10.1021/jf991205y.
A method is described for the analysis of the 16 polycyclic aromatic hydrocarbons (PAHs) prioritized by the USA EPA in meat sausages grilled under common barbecue practices. Quantification was done by GC-MS using perdeuterated internal standards (IS). Validation was done by spiking the matrix at the 0.5 and 1.0 microg/kg levels. The average of expected values ranged from 60 to 134% (median 84%) at the 0.5 microg/kg level and from 69 to 121% (median 96%) at the 1.0 microg/kg level. The median of the limits of detection and quantification were 0.06 and 0.20 microg/kg, respectively, for a 4-g test portion. The carcinogenic PAHs were below the quantification limit in all products except one lamb sausage. Comparison of estimates when either 1, 5, or 16 perdeuterated PAHs were used as IS showed that the most accurate determination of PAHs required that each compound be quantified against its corresponding perdeuterated analogue.
本文描述了一种分析方法，用于测定美国环境保护局（EPA）优先关注的16种多环芳烃（PAHs），这些多环芳烃存在于按照常见烧烤方式烤制的肉香肠中。采用气相色谱 - 质谱联用仪（GC - MS）并使用氘代内标（IS）进行定量分析。通过在基质中添加0.5和1.0微克/千克水平的样品进行方法验证。在0.5微克/千克水平下，预期值的平均值范围为60%至134%（中位数为84%）；在1.0微克/千克水平下，预期值的平均值范围为69%至121%（中位数为96%）。对于4克测试样品，检测限和定量限的中位数分别为0.06和0.20微克/千克。除了一根羊肉香肠外，所有产品中的致癌多环芳烃均低于定量限。当分别使用1种、5种或16种氘代多环芳烃作为内标进行估计比较时，结果表明，要最准确地测定多环芳烃，每种化合物都需要针对其相应的氘代类似物进行定量。
