Otoi T, Koyama N, Yamamoto K, Horikita N, Tachikawa S, Suzuki T
Department of Veterinary Sciences, Yamaguchi University, Japan.
Vet J. 2000 May;159(3):282-6. doi: 10.1053/tvjl.1999.0408.
Two-hundred-and-thirty-one fair-quality embryos at the compacted morula stage collected from 89 superovulated cows were cultured in TCM199 or Brinster's BMOC-3 medium with or without 100 microM beta-mercaptoethanol (beta-ME). After 24 h culture, a total of 142 fair-quality embryos developed to the blastocyst stage, of which 106 were subsequently frozen with 1.8 M ethylene glycol. The mean cell number and development rates of frozen-thawed blastocysts from the fair-quality embryos cultured in TCM199 containing beta-ME were higher than those of the fair-quality embryos directly frozen without culture. The pregnancy rates obtained with frozen blastocysts from fair-quality embryos tended to be lower than those of non-cultured fresh fair-quality embryos and cultured fresh blastocysts. These results indicate that the inclusion of beta-ME in pre-freezing culture media improve the development of frozen-thawed blastocysts from fair-quality embryos, but not the pregnancy rate.
从89头超排奶牛收集的231枚致密桑葚胚期的优质胚胎，在含或不含100微摩尔β-巯基乙醇（β-ME）的TCM199或布林斯特氏BMOC-3培养基中培养。培养24小时后，共有142枚优质胚胎发育至囊胚期，其中106枚随后用1.8 M乙二醇冷冻。在含β-ME的TCM199中培养的优质胚胎冻融后囊胚的平均细胞数和发育率高于未经培养直接冷冻的优质胚胎。优质胚胎冻融囊胚的妊娠率往往低于未培养的新鲜优质胚胎和培养后的新鲜囊胚。这些结果表明，冷冻前培养基中添加β-ME可改善优质胚胎冻融后囊胚的发育，但不能提高妊娠率。
